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Expression data from the mushroom bodies of Drosophila-PKA activity dependent manner

ABSTRACT: Temperature preference behavior in Drosophila depends on the level of PKA signaling in the mushroom bodies. To identify new components downstream to PKA, we carried out a genome-wide screen for genes regulated by PKA signaling in the mushroom bodies. Using the Gal4-UAS system, we increased or decreased PKA activity in the mushroom bodies by expressing dominant-negative (UAS-PKADN) or constitutively active PKA (UAS-PKACA), respectively. Expression of PKA transgenes was targeted to the mushroom bodies using the mushroom body-specific MB247-Gal4 driver. PKA expression was induced for 12-16 hours in three-day-old adults by inactivating the temperature-sensitive Gal80 at the restrictive temperature. We then analyzed gene-expression profiles to identify the genes showing altered expression levels in response to the high or low PKA activity.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Jongkyun Kang

PROVIDER: E-GEOD-28181 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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