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Comparative analysis of gene expression changes upon Cabut or dE2F1/dDP overexpression in the Drosophila wing

ABSTRACT: Investigation of whole genome gene expression level changes in dissected Drosophila wings at the wandering L3 larval stage, 24h after pupa formation and 36h after pupa formation, expressing either UAS-Cabut or UAS-dE2F1 + UAS-dDP under control of apterous-gal4 with a tubulin driven temperature-sensitive gal80 transgene, compared to the parental strain lacking UAS transgenes.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE30484 | GEO | 2011/07/08

SECONDARY ACCESSION(S): PRJNA143475

REPOSITORIES: GEO

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Comparative analysis of gene expression changes upon Cabut or dE2F1/dDP overexpression in the Drosophila wing

Project description:Investigation of whole genome gene expression level changes in dissected Drosophila wings at the wandering L3 larval stage, 24h after pupa formation and 36h after pupa formation, expressing either UAS-Cabut or UAS-dE2F1 + UAS-dDP under control of apterous-gal4 with a tubulin driven temperature-sensitive gal80 transgene, compared to the parental strain lacking UAS transgenes. A 36-chip study using total RNA recovered from four independently isolated samples of 10 dissected Drosophila wings at the indicated developmental stages expressing the indicated UAS-transgenes or the non-UAS-containing matched control strain. One sample (2D) did not pass our internal quality control and, therefore, was not included in the analysis. The dE2F1+dDP data included here is further discussed in L. Buttitta, A.J. Katzaroff, B.A. Edgar (2010). A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo. Journal of Cell Biology vol. 189, 981-96 (PMID 20548101). The Cabut data included here is further discussed in A.J. Katzaroff et al. (2011). The Krüppel-like-factor cabut has cell cycle regulatory properties similar to E2F.

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Project description:Investigation of whole genome gene expression level changes in dissected Drosophila wings of the genotype w1118, at the wandering L3 larval stage, 2h, 6h, 18h, 24h and 36h After pupa formation. Stages after pupa formation were compared to the larval L3 stage to identify changes in gene expression. The data included here is further discussed in O’Keefe, Thomas, Edgar and Buttitta (2012). Combinatorial control of temporal gene expression in the Drosophila wing by enhancers and core promoters Submitted to BMC Genomics A 24 chip study using total RNA recovered from three to four independently isolated samples of 10 dissected Drosophila wings at the indicated developmental stages from flies with the genotype w1118;+;+ (Bloomington Stock #3605). For sample 36BD, total RNA from sample 36B and 36D were pooled, labeled and hybridized independently.

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