Dataset Information

Gel based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire

ABSTRACT: Plasma membrane (PM) encompasses total cellular contents, serves as a living barrier regulates all cellular exchanges in a spatiotemporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous components, which make the PM protein repertoire to be diverse and dynamic. Here, we report the systematic analysis of PM proteome of a food legume, chickpea and have developed a PM proteome reference map. Proteins were extracted from highly enriched PM fractions using aqueous two-phase partitioning. To address a population of PM proteins as comprehensive as possible, both gel-based and gel-free approaches were employed, which led to the identification of a set of 2754 non-redundant proteins. These included both integral membrane proteins having bilayer spanning domains as well as peripheral proteins associated with PMs through protein-protein interactionsor post-translational modifications.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Cicer Arietinum (chickpea) (garbanzo)

TISSUE(S): Shoot

SUBMITTER: Niranjan Chakraborty

LAB HEAD: Dr. Niranjan Chakraborty

PROVIDER: PXD003697 | Pride | 2024-12-05

REPOSITORIES: Pride

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Gel-based and gel-free search for plasma membrane proteins in chickpea (Cicer arietinum L.) augments the comprehensive data sets of membrane protein repertoire.

Barua Pragya P Subba Pratigya P Lande Nilesh Vikram NV Mangalaparthi Kiran K KK Prasad T S Keshava TSK Chakraborty Subhra S Chakraborty Niranjan N

Journal of proteomics 20160422

<h4>Unlabelled</h4>Plasma membrane (PM) encompasses total cellular contents, serving as semi-porous barrier to cell exterior. This living barrier regulates all cellular exchanges in a spatio-temporal fashion. Most of the essential tasks of PMs including molecular transport, cell-cell interaction and signal transduction are carried out by their proteinaceous components, which make the PM protein repertoire to be diverse and dynamic. Here, we report the systematic analysis of PM proteome of a food ...[more]

PMID: 27109347

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Project description:Inflammation is the natural defensive response of the immune system to an injury or infection and is regulated by small molecule mediators triggering different phases of the inflammatory process. In particular, lipid mediators (LM) and cytokines exhibit crucial regulatory functions in the progression and resolution of inflammation. Macrophages play a central role in this process and can adopt distinct phenotypes with specialized functions depending on their microenvironment: inflammatory M1 macrophages drive inflammation by the release of pro-inflammatory cytokines and LMs, like prostaglandins (PG) and leukotrienes (LT), while resolving M2 macrophages promote inflammation resolution and tissue regeneration by production of anti-inflammatory cytokines and specialized pro resolving mediators (SPM). Aging is associated with chronic and unresolved, low-grade inflammation (“inflammaging”) and aging-related dysfunction of macrophages in the resolution of inflammation and tissue maintenance has been reported. Yet, the underlying molecular mechanisms and functional consequences of latter processes remain poorly understood. Here, we show that polarization of peritoneal macrophages (PM) from geriatric mice towards an M2-like phenotype is impaired versus adult mice, resulting in aberrant LM formation and cytokine release. In PMs isolated from adult mice (PM-A) we observed a shift in LM formation from PGs and LTs to SPMs already after 4 h of polarization towards M2 with interleukin (IL) 4. In contrast, PMs from geriatric mice (PM-G) produced mainly LTs and PGs upon polarization. This pattern persists over the course of 48 h of polarization. Proteomic profiling revealed that polarization of PM A towards M2 yields a more distinct phenotype, clearly separated from M1, when compared to PM-G. We observed similar aging-related changes in the lipidome and cytokine profile of spleen, lung and liver tissue from mice. Hence, we hypothesize that during aging macrophage polarization towards M2 is impaired, which in turn drives chronic inflammation and disturbs tissue maintenance. By combining state-of-the art lipidomic and proteomic profiling we aim to uncover new molecular targets for pharmaceutical interventions to improve therapeutic strategies for elderly patients with chronic inflammatory diseases.

			Action	DRS
	Chickpea_In_gel.msf	Msf
	Chickpea_In_gel_1.raw	Raw
	Chickpea_In_gel_2.raw	Raw
	Chickpea_In_gel_3.raw	Raw
	Chickpea_In_gel_4.raw	Raw