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Expression data from Ly6G+ and Ly6G- dendritic cells (DC)

ABSTRACT: To investigate the functional properties of Ly6G+ DC, we employed GeneChip analysis to compare the gene expression profiles between Ly6G+ DC and Ly6C- DC. Crude bone marrow (BM) cells prepared from C57BL/6 mice were cultured in the presence of GM-CSF. On day 6, CD11c+/MHC II+/Ly6G+ cells and CD11c+/MHC II+/Ly6G- cells were simultaneously sorted from the same cultures by FACSAria. Total RNA were extracted and hybridized on Affymetrix microarrays.

ORGANISM(S): Mus musculus

SUBMITTER: Hironori Matsushima

PROVIDER: E-GEOD-28408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Neutrophil differentiation into a unique hybrid population exhibiting dual phenotype and functionality of neutrophils and dendritic cells.

Matsushima Hironori H Geng Shuo S Lu Ran R Okamoto Takashi T Yao Yi Y Mayuzumi Nobuyasu N Kotol Paul F PF Chojnacki Benjamin J BJ Miyazaki Toru T Gallo Richard L RL Takashima Akira A

Blood 20130110 10

Neutrophils have been reported to acquire surface expression of MHC class II and co-stimulatory molecules as well as T-cell stimulatory activities when cultured with selected cytokines. However, cellular identity of those unusual neutrophils showing antigen presenting cell (APC)-like features still remains elusive. Here we show that both immature and mature neutrophils purified from mouse bone marrow differentiate into a previously unrecognized "hybrid" population showing dual properties of both ...[more]

PMID: 23305731

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