Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling time series of Rattus norvegicus liver and kidney samples from animals treated with ochratoxin A and controls

ABSTRACT: Ochratoxin A gene expression profiling in liver and kidney, with time points of exposure from 7 days to 12 months Experiment Overall Design: three replicates for each timepoint measured, control and ochratoxin A treated for both liver and kidney.

Note that files GSM62732.CEL and GSM62733.CEL, and files GSM62730.CEL and GSM62739.CEL as downloaded from GEO contain identical data.

ORGANISM(S): Rattus norvegicus

SUBMITTER: James Holzwarth

PROVIDER: E-GEOD-2852 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A toxicogenomics approach to identify new plausible epigenetic mechanisms of ochratoxin a carcinogenicity in rat.

Marin-Kuan M M Nestler S S Verguet C C Bezençon C C Piguet D D Mansourian R R Holzwarth J J Grigorov M M Delatour T T Mantle P P Cavin C C Schilter B B

Toxicological sciences : an official journal of the Society of Toxicology 20051026 1

Ochratoxin A (OTA) is a mycotoxin occurring naturally in a wide range of food commodities. In animals, it has been shown to cause a variety of adverse effects, nephrocarcinogenicity being the most prominent. Because of its high toxic potency and the continuous exposure of the human population, OTA has raised public health concerns. There is significant debate on how to use the rat carcinogenicity data to assess the potential risk to humans. In this context, the question of the mechanism of actio ...[more]

PMID: 16251485

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Project description:A series of two color gene expression profiles obtained using Agilent 44K expression microarrays was used to examine sex-dependent and growth hormone-dependent differences in gene expression in rat liver. This series is comprised of pools of RNA prepared from untreated male and female rat liver, hypophysectomized (‘Hypox’) male and female rat liver, and from livers of Hypox male rats treated with either a single injection of growth hormone and then killed 30, 60, or 90 min later, or from livers of Hypox male rats treated with two growth hormone injections spaced 3 or 4 hr apart and killed 30 min after the second injection. The pools were paired to generate the following 6 direct microarray comparisons: 1) untreated male liver vs. untreated female liver; 2) Hypox male liver vs. untreated male liver; 3) Hypox female liver vs. untreated female liver; 4) Hypox male liver vs. Hypox female liver; 5) Hypox male liver + 1 growth hormone injection vs. Hypox male liver; and 6) Hypox male liver + 2 growth hormone injections vs. Hypox male liver. A comparison of untreated male liver and untreated female liver liver gene expression profiles showed that of the genes that showed significant expression differences in at least one of the 6 data sets, 25% were sex-specific. Moreover, sex specificity was lost for 88% of the male-specific genes and 94% of the female-specific genes following hypophysectomy. 25-31% of the sex-specific genes whose expression is altered by hypophysectomy responded to short-term growth hormone treatment in hypox male liver. 18-19% of the sex-specific genes whose expression decreased following hypophysectomy were up-regulated after either one or two growth hormone injections. Finally, growth hormone suppressed 24-36% of the sex-specific genes whose expression was up-regulated following hypophysectomy, indicating that growth hormone acts via both positive and negative regulatory mechanisms to establish and maintain the sex specificity of liver gene expression. For full details, see V. Wauthier and D.J. Waxman, Molecular Endocrinology (2008)

Dataset Information

Transcription profiling time series of Rattus norvegicus liver and kidney samples from animals treated with ochratoxin A and controls

Publications

A toxicogenomics approach to identify new plausible epigenetic mechanisms of ochratoxin a carcinogenicity in rat.

Similar Datasets

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