Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling by array of mouse kidneys following chronic ACTH treatment or control treatment

ABSTRACT: This experiment compares gene expression in kidneys of control and ACTH treated mice.
Kidney RNA was prepared from 6 mice: three controls and three ACTH treated and processed by the microarray team at Ark Genomics and the Roslin Institute.
RNAs were processed through standard Affymetrix protocols, with one round of cDNA amplification. Processed RNAs were hybridised to Affymetrix Mouse Genome 430 2.0 GeneChip, and data were extracted through the GCOS software.
CEL files were made available for further data processing via RMA in Bioconductor.

ORGANISM(S): Mus musculus

SUBMITTER: Donald Dunbar

PROVIDER: E-MEXP-1761 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney.

Dunbar Donald R DR Khaled Hiba H Evans Louise C LC Al-Dujaili Emad A S EA Mullins Linda J LJ Mullins John J JJ Kenyon Christopher J CJ Bailey Matthew A MA

Physiological genomics 20091117 3

We investigated the effects on urinary steroid and electrolyte excretion and renal gene expression of chronic infusions of ACTH in the mouse. ACTH caused a sustained increase in corticosteroid excretion; aldosterone excretion was only transiently elevated. There was an increase in the excretion of deoxycorticosterone, a weak mineralocorticoid, to levels of physiological significance. Nevertheless, we observed neither antinatriuresis nor kaliuresis in ACTH-treated mice, and plasma renin activity ...[more]

PMID: 19920212

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Dataset Information

Transcription profiling by array of mouse kidneys following chronic ACTH treatment or control treatment

Publications

Transcriptional and physiological responses to chronic ACTH treatment by the mouse kidney.

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