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Unknown,Transcriptomics,Genomics,Proteomics

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Expression profiles of human primary keratinocytes differentiated with TPA

ABSTRACT: Human primary keratinocytes were left untreated or treated for 48 hr with 12-O-tetradecanoylphorbol-13-acetate (TPA) and expression profiles were compared to find long ncRNAs expressed and differentially regulated upon differentiation. 4 replicates of minus TPA and 3 of plus TPA with dye-swap. Custom-made microarray from Agilent.

ORGANISM(S): Homo sapiens

SUBMITTER: Ulf Ørom

PROVIDER: E-GEOD-28552 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Long noncoding RNAs with enhancer-like function in human cells.

Ørom Ulf Andersson UA Derrien Thomas T Beringer Malte M Gumireddy Kiranmai K Gardini Alessandro A Bussotti Giovanni G Lai Fan F Zytnicki Matthias M Notredame Cedric C Huang Qihong Q Guigo Roderic R Shiekhattar Ramin R

Cell 20101001 1

While the long noncoding RNAs (ncRNAs) constitute a large portion of the mammalian transcriptome, their biological functions has remained elusive. A few long ncRNAs that have been studied in any detail silence gene expression in processes such as X-inactivation and imprinting. We used a GENCODE annotation of the human genome to characterize over a thousand long ncRNAs that are expressed in multiple cell lines. Unexpectedly, we found an enhancer-like function for a set of these long ncRNAs in hum ...[more]

PMID: 20887892

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Expression profiles of human primary keratinocytes differentiated with TPA

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