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Expression data of the human hepatoblastoma cell line HepG2 treated with TGF-beta

ABSTRACT: Smad2/3 are transcription factors that engage in TGF-beta-induced transcription. We determined and analyzed HepG2 and Hep3B-specific Smad2/3 binding sites by ChIP-chip. We used expression microarrays to compare the Smad2/3 and HNF4alpha binding sites identified by ChIP-chip or ChIP-seq, respectively, to TGF-beta-induced gene expressions. HepG2 cells were transfected with control or HNF4A siRNAs and treated with 3 ng/ml TGF-beta for 0, 1.5 and 24 h (6 samples in total, no replicates). Total RNA was extracted and expression microarray analysis was performed as described in the protocols.

ORGANISM(S): Homo sapiens

SUBMITTER: Daizo Koinuma

PROVIDER: E-GEOD-28590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cell type-specific target selection by combinatorial binding of Smad2/3 proteins and hepatocyte nuclear factor 4alpha in HepG2 cells.

Mizutani Anna A Koinuma Daizo D Tsutsumi Shuichi S Kamimura Naoko N Morikawa Masato M Suzuki Hiroshi I HI Imamura Takeshi T Miyazono Kohei K Aburatani Hiroyuki H

The Journal of biological chemistry 20110606 34

Specific regulation of target genes by transforming growth factor-β (TGF-β) in a given cellular context is determined in part by transcription factors and cofactors that interact with the Smad complex. In this study, we determined Smad2 and Smad3 (Smad2/3) binding regions in the promoters of known genes in HepG2 hepatoblastoma cells, and we compared them with those in HaCaT epidermal keratinocytes to elucidate the mechanisms of cell type- and context-dependent regulation of transcription induced ...[more]

PMID: 21646355

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