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Genome-wide location analysis of BMP (SMAD1) in mouse erythroid progenitors co-occupted with lineage specific regulators (GATA1, GATA2)

ABSTRACT: Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration Mouse G1E or G1ER cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total Smad1 (Santa Cruz SC-7965), Gata1 (Santa Cruz SC-265), or Gata2 (Santa Cruz SC-9008). This represents the Mouse ChIP-seq portion of this dataset.

ORGANISM(S): Mus musculus

SUBMITTER: Teresa Bowman

PROVIDER: E-GEOD-29193 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Lineage regulators direct BMP and Wnt pathways to cell-specific programs during differentiation and regeneration.

Trompouki Eirini E Bowman Teresa V TV Lawton Lee N LN Fan Zi Peng ZP Wu Dai-Chen DC DiBiase Anthony A Martin Corey S CS Cech Jennifer N JN Sessa Anna K AK Leblanc Jocelyn L JL Li Pulin P Durand Ellen M EM Mosimann Christian C Heffner Garrett C GC Daley George Q GQ Paulson Robert F RF Young Richard A RA Zon Leonard I LI

Cell 20111001 3

BMP and Wnt signaling pathways control essential cellular responses through activation of the transcription factors SMAD (BMP) and TCF (Wnt). Here, we show that regeneration of hematopoietic lineages following acute injury depends on the activation of each of these signaling pathways to induce expression of key blood genes. Both SMAD1 and TCF7L2 co-occupy sites with master regulators adjacent to hematopoietic genes. In addition, both SMAD1 and TCF7L2 follow the binding of the predominant lineage ...[more]

PMID: 22036566

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