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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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ChIP-Seq of human MCF7 and HEPG2 cells using antibodies against CTCF, STAG1, RAD2, ERa and CEBPa

ABSTRACT: ChIP-Seq study in human MCF7 and HEPG2 cells using antibodies against CTCF (Millipore, 07-729), STAG1 (abcam, ab4457), RAD21 (abcam, ab992), ERa (santa cruz, sc-543), CEBPa (santa cruz, sc-9314)

ORGANISM(S): Homo sapiens

SUBMITTER: Petra Catalina Schwalie

PROVIDER: E-TABM-828 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A CTCF-independent role for cohesin in tissue-specific transcription.

Schmidt Dominic D Schwalie Petra C PC Ross-Innes Caryn S CS Hurtado Antoni A Brown Gordon D GD Carroll Jason S JS Flicek Paul P Odom Duncan T DT

Genome research 20100310 5

The cohesin protein complex holds sister chromatids in dividing cells together and is essential for chromosome segregation. Recently, cohesin has been implicated in mediating transcriptional insulation, via its interactions with CTCF. Here, we show in different cell types that cohesin functionally behaves as a tissue-specific transcriptional regulator, independent of CTCF binding. By performing matched genome-wide binding assays (ChIP-seq) in human breast cancer cells (MCF-7), we discovered thou ...[more]

PMID: 20219941

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[E-TABM-828] ChIP-Seq in human MCF7 and HEPG2 cells

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