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The effects of knockdown of PML by different individual siRNAs in HUVEC cells

ABSTRACT: To study the potential target genes regulated by PML in endothelial cells, we carried out siRNA-mediated knockdown of PML in HUVEC cells. To eliminate the off-target effects of siRNAs, we utilized two different siRNAs. Only the genes changed in the same pattern following both siRNAs transfection are considered as potential PML-knockdown responsive genes. The experiment is one-factor (siRNA) with three ranks (siCtrl, siPML-1, siPML-2). We included technical duplicates for each sample. To minimize systemic errors assoicated with technique, we distributed the duplicates on two different microarray chips.

ORGANISM(S): Homo sapiens

SUBMITTER: xiwen cheng

PROVIDER: E-GEOD-29238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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