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Raf/MEK signatures in zebrafish liver cells

ABSTRACT: An inducible oncogenic cell line ZFL-?Raf1-ER was established, in which oncogenic human Raf-1(?Raf1) can be activated in zebrafish liver cells by administration of 4-hydroxytamoxifen (4HT). Transcriptional profilling of the ZFL-?Raf1-ER cells with and without administration of 4HT and/or the MEK inhibitor U0126 defined the gene signatures transcriptionally regulated by hyperactive Raf/MEK/MAPK signaling in zebrafish liver cells. The ZFL-?Raf1-ER cells were cultured in 12-well plates till confluence. Cell were starved for 4h in plain medium and susequently treated with or without 1µM 4HT and/or 30µM U0126 for 12h. Biological triplications were taken for each condition.

ORGANISM(S): Danio rerio

SUBMITTER: Herman Spaink

PROVIDER: E-GEOD-29308 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Raf/MEK signatures in zebrafish liver cells

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