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Molecular Mechanisms of Bortezomib Resistant Adenocarcinoma cells [CGH data]

ABSTRACT: Copy number and Gene expression profiling of HT-29 wild-type and bortezomib resistant cell lines Identification of mechanisms of bortezomib resistance Copy number differences between HT-29 cell line variants and a HapMap control population of 9 female samples were identified using the Agilent 1M Human CGH microarray

ORGANISM(S): Homo sapiens

SUBMITTER: Jonathan Keats

PROVIDER: E-GEOD-29711 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular Mechanisms of Bortezomib Resistant Adenocarcinoma cells [GEP data]

Project description:Copy number and Gene expression profiling of HT-29 wild-type and bortezomib resistant cell lines Identification of mechanisms of bortezomib resistance Gene expression differences between HT-29 cell line variants

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Molecular Mechanisms of Bortezomib Resistant Adenocarcinoma cells

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RNA-seq analysis of HT-29, MCF10A, and MDA-MB-436 cells

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HT-29 cells treated with IFN-γ

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Human colon cancer HT-29 and LS174T cells: stably transfected with shRNA-Ascl2/EGFP vs. stably transfected with shRNA-Control/EGFP

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Mitochondria help avoid glucose addiction in cancer cells

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2010-05-28 | E-GEOD-16157 | biostudies-arrayexpress

A Salmonella-encoded microRNA-like RNA facilitates bacterial invasion and intracellular replication via suppressing host cell inducible nitric oxide synthase

Project description:As the leading cause of food-borne illness in the world, Salmonella have evolved a sophisticated machinery to alter host cell function to promote virulence and survival.In this study, we compare production of non-coding RNAs between Salmonella-infected cells and mock infection cells. Using Solexa deep sequencing, we detected a panel of 19-24nt Salmonella-derived non-coding RNA fragments with considerable large copy numbers in human colonic epithelial HT-29 cells following Salmonella infection.The fragment with the highest copy number, Sal-1, was further validated by both quantitative reverse transcription polymerase chain reaction (qRT-PCR) and northern blot. The generation of Sal-1 requires the infection of host cells by Salmonella, and the processing of the Sal-1 M-bM-^@M-^\primaryM-bM-^@M-^] or M-bM-^@M-^\precursorM-bM-^@M-^] to the mature Sal-1 in Salmonella-infected cells is Dicer-independent but Argonaute 2 (Ago2)-dependent. Functionally, Sal-1 suppresses the expression of colonic epithelial cell endogenous inducible nitric oxide synthase (iNOS) via targeting its open reading frame and thus reduces the bacterial resistance of host cells. Screening and identification of Salmonella-encoded microRNA-like RNA fragments

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Molecular Mechanisms of Bortezomib Resistant Adenocarcinoma cells [GEP data]

Project description:Copy number and Gene expression profiling of HT-29 wild-type and bortezomib resistant cell lines Identification of mechanisms of bortezomib resistance

2011-06-04 | GSE29712 | GEO

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