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Study of the role of miR-205 in prostate epithelial cells

ABSTRACT: Gene expression has been assessed on RWPE-1 cells, derived from normal prostate, upon silencing of miR-205 by an antisense locked nucleic acid. RWPE-1 cells were transfected in triplicate either with 100nM of an antisense locked nucleic acid targeting miR-205 (LNA-205) or a scrambled oligomer (LNA-Scr). Gene expression was assessed 72h after transfection.

ORGANISM(S): Homo sapiens

SUBMITTER: Paolo Gandellini

PROVIDER: E-GEOD-29782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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miR-205 regulates basement membrane deposition in human prostate: implications for cancer development.

Gandellini P P Profumo V V Casamichele A A Fenderico N N Borrelli S S Petrovich G G Santilli G G Callari M M Colecchia M M Pozzi S S De Cesare M M Folini M M Valdagni R R Mantovani R R Zaffaroni N N

Cell death and differentiation 20120504 11

The basement membrane (BM) is a layer of specialized extracellular matrix that surrounds normal prostate glands and preserves tissue integrity. Lack or discontinuity of the BM is a prerequisite for tumor cell invasion into interstitial spaces, thus favoring metastasis. Therefore, BM maintenance represents a barrier against cancer development and progression. In the study, we show that miR-205 participates in a network involving ΔNp63α, which is essential for maintenance of the BM in prostate epi ...[more]

PMID: 22555458

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