Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

The role of HIF in FH-associated renal neoplasia

ABSTRACT: Fumarate hydratase (FH) mutations predispose to renal cysts cancer. These cancers overexpress hypoxia-inducible factor-alpha (Hif-1a). We have generated a conditional Fh1 (mouse FH) knockout mice that develop renal cysts and overexpress Hif-1a. In order to identify the contribution of Hif-1a to cyst formation we have intercrossed our mice with conditional HIf-1a KO mice. We intercrossed Fh1/Hif1a mice with kidney specific cre recombinase (Ksp-Cre) and analysed kidney cyst formation. RNA was extracted from cysts from 4xFh1 KO, 4xFh1/Hif-1a KO and 4 control mice. For each comparison littermates were used and the animals were aged 15 weeks i.e. early cystic disease.

ORGANISM(S): Mus musculus

SUBMITTER: Natasha Sahgal 

PROVIDER: E-GEOD-29988 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

The Krebs cycle enzyme fumarate hydratase (FH) is a human tumor suppressor whose inactivation is associated with the development of leiomyomata, renal cysts, and tumors. It has been proposed that activation of hypoxia inducible factor (HIF) by fumarate-mediated inhibition of HIF prolyl hydroxylases drives oncogenesis. Using a mouse model, we provide genetic evidence that Fh1-associated cyst formation is Hif independent, as is striking upregulation of antioxidant signaling pathways revealed by ge  ...[more]

Similar Datasets

Transcriptomics The role of HIF in FH-associated renal neoplasia
2011-10-24 | GSE29988 | GEO
Transcriptomics Expression data of cystic renal epithelial tissue from mice deficient for fumarate hydratase.
2010-12-22 | GSE10989 | GEO
Transcriptomics Expression data of cystic renal epithelial tissue from mice deficient for fumarate hydratase.
2010-12-22 | E-GEOD-10989 | biostudies-arrayexpress
Genomics HIRA loss transforms FH deficient cells [ChIP-Seq]
2022-10-29 | GSE203056 | GEO
Genomics
| PRJNA1077720 | ENA
Transcriptomics Fumarate induces vesicular release of mtDNA to drive innate immunity
2023-02-17 | GSE183745 | GEO
Transcriptomics Targeting Krebs-cycle-deficient renal cell carcinoma with Poly ADP-Ribose Polymerase inhibitors and low-dose alkylating chemotherapy
2022-04-21 | GSE201008 | GEO
Transcriptomics RNA-seq on enriched cardiac fibroblasts from the hearts of Hif-1a conditional knockout or heterozygous mice
2021-11-11 | E-MTAB-9674 | biostudies-arrayexpress
Transcriptomics Global genomic profiling of HIF-1a-regulated genes in bone marrow B cells from OVX mice
2022-02-24 | GSE163704 | GEO
Transcriptomics Single-cell sequecing of LCMV-specific WT and HIF-1a-deficient CD8 T cells isolated from LCMV-Clone13 infected host mice
2023-09-22 | GSE213847 | GEO