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Gene expression in pif3 mutant under dark and in wt under Red 1h and Red 18h

ABSTRACT: PIF3 plays a role as repressor of photomorphogenesis in darkness. To identify PIF3-regulated genes that might be implementing this action, we have performed whole-genome expression analysis in the pif3 mutant. Seeds were surface-sterilized and plated on GM medium without sucrose, stratified at 4ºC in the dark for 4 days, exposed to white light for 3 hours to induce germination, and placed in a growth chamber at 21ºC in darkness for 4 days (D0h) and 4 days and 1 hour (D1h). For Rc treatments seedlings were moved to Rc growth chambers at 21ºC for 1 (R1h) and 18 (R18h) hours.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Elena Monte

PROVIDER: E-GEOD-30030 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Functional profiling identifies genes involved in organ-specific branches of the PIF3 regulatory network in Arabidopsis.

Sentandreu Maria M Martín Guiomar G González-Schain Nahuel N Leivar Pablo P Soy Judit J Tepperman James M JM Quail Peter H PH Monte Elena E

The Plant cell 20111122 11

The phytochrome (phy)-interacting basic helix-loop-helix transcription factors (PIFs) constitutively sustain the etiolated state of dark-germinated seedlings by actively repressing deetiolation in darkness. This action is rapidly reversed upon light exposure by phy-induced proteolytic degradation of the PIFs. Here, we combined a microarray-based approach with a functional profiling strategy and identified four PIF3-regulated genes misexpressed in the dark (MIDAs) that are novel regulators of see ...[more]

PMID: 22108407

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