Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression profile of decidual macrophages and different in vitro M1 and M2 macrophage populations

ABSTRACT: Uterine (decidual) macrophages (M) are believed to play an important role in fetal tolerance during pregnancy, but the factors that regulate them are not known. In this study we investigated the potential role of several factors (M-CSF, GM-CSF, IL-4/13, IL-10, progesterone and estradiol) to drive the differentiation and polarization of human decidual M by using an in vitro M differentiation model. To estimate the similarity of the in vitro differentiated M with decidual M, we used the present gene expression array. The array consisted of 420 M-related genes, including 100 genes highly regulated in decidual M based on the gene expression profile previously reported (Gustafsson et al, 2008, PLoS ONE. 3(4): e2078). The analysis focused on the 100 decidual M-associated genes. Comparative expression analysis of CD14+ cells isolated from blood (3 samples) or decidua (3 samples) from pregnant women, CD14+ cells isolated from non-pregnant women (14 samples) and different types of in vitro-generated macrophages (55 samples). A total of 75 samples were anlayzed.

ORGANISM(S): Homo sapiens

SUBMITTER: Robert Geffers

PROVIDER: E-GEOD-30595 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Macrophages at the fetal-maternal interface express markers of alternative activation and are induced by M-CSF and IL-10.

Svensson Judit J Jenmalm Maria C MC Matussek Andreas A Geffers Robert R Berg Göran G Ernerudh Jan J

Journal of immunology (Baltimore, Md. : 1950) 20110902 7

During pregnancy, the maternal immune system is challenged by the presence of the fetus, which must be tolerated despite being semiallogeneic. Uterine mucosal (or decidual) macrophages (M), one of the major leukocyte populations at the fetal-maternal interface, have been implicated in fetal tolerance, but information regarding their regulation is scarce. In this study, we investigated the role of several factors potentially involved in the differentiation and polarization of decidual M with an i ...[more]

PMID: 21890660

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Project description:miRNA profiling of CD34+ derived, in vitro-generated Langerhans cells (LCs) and interstitial-type dendritic cells (intDCs). Epidermal Langerhans cells (LCs) form a network of dendritic cells (DCs) in basal/suprabasal layers of the skin and are characterized by a unique phenotype (CD207+ CD1abright CD324+ CD11b-). Due to their specific location at body surfaces, LCs are constantly exposed to environmental stimuli. Conversely, interstitial-type DCs (intDCs) are located in adjacent tissues and can be discriminated from LCs phenotypically (CD207- CD1adim CD324- CD11b+). These DCs constitute a second line of defense against pathogens that have crossed the epithelial barrier. During development both DC subsets originally arise from myeloid progenitor cells via monocyte-committed intermediates in response to specific microenvironmental signals. Additionally certain pools of DCs can be replenished by tissue resident cells or monocytes in response to specific microenvironmental signals (2, 4, 5). In vitro generated GM-CSF/IL-4-dependent DCs derived either from CD14+ monocytes or via CD14+CD11b+ monocyte intermediates from CD34+ progenitor cells share many characteristics with intDCs in vivo. On the other hand, LCs generated from CD34+ cells under serum-free TGF-beta1-dependent conditions phenotypically resemble epidermal-resident LCs. Since these two DC subsets are of considerable interest for clinical cell therapy studies, an improved understanding of their development and function is of substantial relevance. To identify differentially expressed miRNAs in DC subsets, we performed a miRNA screen. Therefore, we generated LCs or intDCs from human CD34+ cord blood haematopoietic progenitor cells as described. For intDCs, CD34+ cells (1 x 104 to 2 x 104/ml per well) were cultured in 24-well tissue culture plates in serum free CellGroÂ® DC medium (CellGenix, Freiburg, Germany) supplemented with GM-CSF (100 ng/ml), SCF (20 ng/ml), FL (50 ng/ml) and TNFalpha (2.5 ng/ml) for 3-5 days and subsequently with GM-CSF (100 ng/ml) and IL-4 (2.5 ng/ml) for 4-5 days. For LCs, CD34+ cells (1 x 104 to 2 x 104/ml per well) were cultured in 24-well tissue culture plates in serum free CellGroÂ® DC medium (CellGenix, Freiburg, Germany) supplemented with GM-CSF (100 ng/ml), SCF (20 ng/ml), FL (50 ng/ml), TNFalpha (2.5 ng/ml) and TGF-beta1 (0.5 ng/ml). The well-established immunophenotype of LCs (CD1ahi CD11b- CD207+ CD324+) and intDCs (CD1a+ CD11b+ CD207- CD324-) was confirmed by FACS. These two DC subsets were purified and submitted to differential miRNA profiling. Two conditioned experiment LCs vs. intDCs. Biological replicates: 3 LCs, 3 intDCs, independently differentiated, harvested and purified. 3 replicates each were pooled and hybridized on one array. Supplementary files linked below.

Dataset Information

Gene expression profile of decidual macrophages and different in vitro M1 and M2 macrophage populations

Publications

Macrophages at the fetal-maternal interface express markers of alternative activation and are induced by M-CSF and IL-10.

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