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ChIP-chip from 48h hemin induced K562 cells with GATA-1, EKLF, and NF-E2 antibodies

ABSTRACT: The transcription factor GATA-1, EKLF and NF-E2 promotes erythroid differentition by regulating their target genes, however, the intricate interplays between these key TFs and microRNA genes are largely unknown. Chromatin immunoprecipitation (ChIP) of GATA-1, EKLF and NF-E2 together with microRNA genomic promoter profiling by ChIP-on-chip analysis demonstrated that GATA-1, EKLF and NF-E2 collaborately regulate a series of microRNA genes. Comparison of microRNA promoter arrays of GATA-1 VS EKLF VS NF-E2 in K562 cells suffering with hemin induced erythroid differentiation

ORGANISM(S): Homo sapiens

SUBMITTER: Jia Yu

PROVIDER: E-GEOD-30808 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systematical identification of GATA-1, EKLF, and NF-E2 regulated miRNAs by ChIP-on chip and microRNA microarray in K562 cells into erythroid differentiation

Project description:This SuperSeries is composed of the following subset Series: GSE30380: Expression profile of hemin induced erythroid differentiation, over-expressing and knocking-down GATA-1, EKLF and NF-E2 in K562s GSE30808: ChIP-chip from 48h hemin induced K562 cells with GATA-1, EKLF, and NF-E2 antibodies Refer to individual Series

2011-12-31 | E-GEOD-30809 | biostudies-arrayexpress

ChIP-chip from 48h hemin induced K562 cells with GATA-1, EKLF, and NF-E2 antibodies

Project description:The transcription factor GATA-1, EKLF and NF-E2 promotes erythroid differentition by regulating their target genes, however, the intricate interplays between these key TFs and microRNA genes are largely unknown. Chromatin immunoprecipitation (ChIP) of GATA-1, EKLF and NF-E2 together with microRNA genomic promoter profiling by ChIP-on-chip analysis demonstrated that GATA-1, EKLF and NF-E2 collaborately regulate a series of microRNA genes.

2011-12-31 | GSE30808 | GEO

Expression profile of hemin induced erythroid differentiation, over-expressing and knocking-down GATA-1, EKLF and NF-E2 in K562s

Project description:we profiled miRNA gene expression with the Illumina hybridization system in K562 cells induced by hemin and K562 cells with over-expressing or knocking-down GATA-1, EKLF or NF-E2 treatments. To find differential expression miRNAs, we profiled miRNA gene expression with the Illumina hybridization system in untreated, hemin-treated 48h and 72h K562 cells. To define GATA-1, EKLF or NF-E2 directly targeted miRNA genes, a comprehensive analysis of TF induced miRNA gene expression changes was performed using over-expressing or knocking-down TFs in K562 cells and illumina miRNA profiling Beadchip system.

2011-12-31 | E-GEOD-30380 | biostudies-arrayexpress

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2011-12-31 | GSE30380 | GEO

Homo sapiens

Project description:ChIP-chip from 48h hemin induced K562 cells with GATA-1, EKLF, and NF-E2 antibodies

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Systematical identification of GATA-1, EKLF, and NF-E2 regulated miRNAs by ChIP-on chip and microRNA microarray in K562 cells into erythroid differentiation

Project description:Systematical identification of GATA-1, EKLF, and NF-E2 regulated miRNAs by ChIP-on chip and microRNA microarray in K562 cells into erythroid differentiation

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Homo sapiens

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Genome-wide ChIP-Seq reveals a dramatic shift in the binding of the transcription factor erythroid Kruppel-like factor during erythrocyte differentiation

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Genome-wide ChIP-Seq reveals a dramatic shift in the binding of the transcription factor erythroid Kruppel-like factor during erythrocyte differentiation

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2013-03-30 | E-GEOD-45625 | biostudies-arrayexpress

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