Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Centromere-Like Regions in Budding Yeast

ABSTRACT: Accurate chromosome segregation requires centromeres (CENs), the chromosomal sites where kinetochores form, to bridge DNA and attach to microtubules. In contrast to most eukaryotes, Saccharomyces cerevisiae possesses sequence-defined point centromeres. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) of four kinetochore components reveals regions of overlapping, extra-centromeric protein localization upon overproduction of the centromeric histone, Cse4 (CENP-A or CenH3). These identified sequences enhance proper plasmid and chromosome segregation, and are termed Centromere-like Regions (CLRs). CLRs form in close proximity to S. cerevisiae CENs and share characteristics typical of point and regional centromeres. CLR sequences are conserved among related budding yeasts, suggesting a role in vivo. These studies provide new insights into the origin and evolution of centromeres. ChIP-Seq analysis of the kinetochore components Cse4, Mif2, Ndc10 and Ndc80 in budding yeast strains (Saccharomyces cerevisiae) with normal and elevated levels of Cse4

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Raymond Auerbach 

PROVIDER: E-GEOD-31466 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Centromere-like regions in the budding yeast genome.

Lefrançois Philippe P   Auerbach Raymond K RK   Yellman Christopher M CM   Roeder G Shirleen GS   Snyder Michael M  

PLoS genetics 20130117 1

Accurate chromosome segregation requires centromeres (CENs), the DNA sequences where kinetochores form, to attach chromosomes to microtubules. In contrast to most eukaryotes, which have broad centromeres, Saccharomyces cerevisiae possesses sequence-defined point CENs. Chromatin immunoprecipitation followed by sequencing (ChIP-Seq) reveals colocalization of four kinetochore proteins at novel, discrete, non-centromeric regions, especially when levels of the centromeric histone H3 variant, Cse4 (a.  ...[more]

Similar Datasets

Genomics Genomewide Localization of Yeast CENP-A (Cse4) in Wild-type, met30-6, and cdc4-1 Strains
2019-04-03 | GSE129195 | GEO
Genomics Psh1-mediated Proteolysis Prevents the Budding Yeast Centromeric Histone Variant from Mislocalizing to Promoter Nucleosomes [ChIP-Seq]
2016-03-08 | GSE69695 | GEO
Transcriptomics Psh1-mediated Proteolysis Prevents the Budding Yeast Centromeric Histone Variant from Mislocalizing to Promoter Nucleosomes [RNA-seq]
2016-03-08 | GSE77596 | GEO
Genomics The budding yeast Centromere DNA Element II wraps a stable Cse4 hemisome in either orientation in vivo
2014-04-15 | E-GEOD-51949 | biostudies-arrayexpress
Genomics Cryptococcus amylolentus
| PRJNA376407 | ENA
Genomics Genome-wide profile of the SWI/SNF chromatin remodeling complex
2011-01-10 | E-GEOD-24397 | biostudies-arrayexpress
Genomics Centromere-Like Regions in Budding Yeast
2013-01-18 | GSE31466 | GEO
Genomics A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress
2009-01-22 | E-GEOD-14283 | biostudies-arrayexpress
Genomics Dbf4-Dependent Kinase (DDK)-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A in Saccharomyces cerevisiae
2020-04-04 | GSE148068 | GEO
Genomics Tripartite organization of centromeric chromatin in budding yeast
2011-12-07 | E-GEOD-28298 | biostudies-arrayexpress