Dataset Information

Intensity-based absolute quantification (iBAQ) of proteins in MDA-MB231

ABSTRACT: To determine the absolute copy number of proteins in MDA-MB231 breast cancer cells, we employed IBAQ mediated absolute quantification of proteins based on (Schwanhäusser et al., Nature, 2011), with some modifications. Maqquant calculated iBAQ values were calibrated using spike-in standards, and used to calculate copy numbers for each identified protein within the dataset. Copy numbers for a total of 3,584 proteins were calculated in MDA-MB231 cells.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Permanent Cell Line Cell, Cell Culture

DISEASE(S): Breast Cancer

SUBMITTER: Faraz Mardakheh

LAB HEAD: Faraz K. Mardakheh

PROVIDER: PXD024139 | Pride | 2021-05-14

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20201016_MADforFaraz_MDALysate_minusStandart_sample1.raw	Raw
	20201016_MADforFaraz_MDALysate_minusStandart_sample1_R2.raw	Raw
	20201016_MADforFaraz_MDALysate_plusStandart_sample2.raw	Raw
	20201016_MADforFaraz_MDALysate_plusStandart_sample2_R2.raw	Raw
	mqpar.xml	Xml

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Publications

A prometastatic splicing program regulated by SNRPA1 interactions with structured RNA elements.

Fish Lisa L Khoroshkin Matvei M Navickas Albertas A Garcia Kristle K Culbertson Bruce B Hänisch Benjamin B Zhang Steven S Nguyen Hoang C B HCB Soto Larisa M LM Dermit Maria M Mardakheh Faraz K FK Molina Henrik H Alarcón Claudio C Najafabadi Hamed S HS Goodarzi Hani H

Science (New York, N.Y.) 20210501 6543

Aberrant alternative splicing is a hallmark of cancer, yet the underlying regulatory programs that control this process remain largely unknown. Here, we report a systematic effort to decipher the RNA structural code that shapes pathological splicing during breast cancer metastasis. We discovered a previously unknown structural splicing enhancer that is enriched near cassette exons with increased inclusion in highly metastatic cells. We show that the spliceosomal protein small nuclear ribonucleop ...[more]

PMID: 33986153

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