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Expression changes in Yy1 knock down mouse embryonic stem cells

ABSTRACT: We have determined the global gene expression upon loss of function of the Yy1 transcription factor in mouse embryonic stem cells Total RNA was extracted from ES cells transiently transfected with yy1-specific or scrampled-control siRNA oligos 48 hr post-transfection.

ORGANISM(S): Mus musculus

SUBMITTER: Diego Pasini

PROVIDER: E-GEOD-31784 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells.

Vella Pietro P Barozzi Iros I Cuomo Alessandro A Bonaldi Tiziana T Pasini Diego D

Nucleic acids research 20111230 8

The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and mediates Polycomb group proteins (PcG) recruitment to DNA. Despite several publications pointing at YY1 having a similar role in mammalians, others showed features of YY1 that are not compatible with P ...[more]

PMID: 22210892

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Yy1 activity in mouse embryonic stem cells

Project description:This SuperSeries is composed of the following subset Series: GSE31784: Expression changes in Yy1 knock down mouse embryonic stem cells GSE31785: Yy1 occupancy of mouse ES cell genome Refer to individual Series

2012-01-04 | E-GEOD-31786 | biostudies-arrayexpress

Ogt chromatin recruitment is mediated by TET proteins in mouse ES cells [expression array]

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2013-01-06 | E-GEOD-39321 | biostudies-arrayexpress

Ogt chromatin recruitment is mediated by TET proteins in mouse ES cells

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2013-01-06 | E-GEOD-39323 | biostudies-arrayexpress

Polycomb proteins link Proliferation with DNA Replication

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2014-04-15 | E-GEOD-48520 | biostudies-arrayexpress

The Histone Methyltransferase Wbp7 Controls Macrophage Function through GPI Glycolipid Anchor Synthesis. [Expression Profile]

Project description:Histone methyltransferases catalyze site-specific deposition of methyl groups, enabling recruitment of transcriptional regulators. In mammals, trimethylation of lysine 4 in histone H3, a modification localized at the transcription start sites of active genes, is catalyzed by six enzymes (SET1a and SET1b, MLL1M-bM-^@M-^SMLL4) whose specific functions are largely unknown. By using a genomic approach, we found that in macrophages, MLL4 (also known as Wbp7) was required for the expression of Pigp, an essential component of the GPI-GlcNAc transferase, the enzyme catalyzing the first step of glycosylphosphatidylinositol (GPI) anchor synthesis. Impaired Pigp expression in Wbp7-/- macrophages abolished GPI anchor-dependent loading of proteins on the cell membrane. Consistently, loss of GPI-anchored CD14, the coreceptor for lipopolysaccharide (LPS) and other bacterial molecules, markedly attenuated LPS-triggered intracellular signals and gene expression changes. These data link a histone-modifying enzyme to a biosynthetic pathway and indicate a specialized biological role for Wbp7 in macrophage function and antimicrobial response. Gene expression profiles for bone marrow-derived macrophages (BMDM) from either Wbp7+/- (HET) or Wbp7-/- (KO). Cells were left untreated or challenged with lipopolysaccharide (LPS) for 2hrs or 4hrs. Each genotype-treatment combination were performed in triplicate.

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HDAC3 requirement for the inflammatory gene expression program in macrophages [gene expression]

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2012-07-08 | E-GEOD-33162 | biostudies-arrayexpress

The Histone Methyltransferase Wbp7 Controls Macrophage Function through GPI Glycolipid Anchor Synthesis

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2012-04-08 | E-GEOD-30973 | biostudies-arrayexpress

Gene expression profiles in Elastin (Eln+/-) mouse aorta and aortic valve tissue

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HDAC3 requirement for the inflammatory gene expression program in macrophages

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2012-07-08 | E-GEOD-33164 | biostudies-arrayexpress

Yy1 occupancy of mouse ES cell genome

Project description:These data include the genome wide location analyses of Yy1 by bioChIPs of Fbio-Yy1. Streptavidin precipitation of formaldehyde cross-linked chromatin prepared from Fbio-Yy1 and control expressing mouse ES cells

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