Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Bedbug RNA-seq

ABSTRACT: A comparison of mRNA profiles of insecticide resistant and susceptible genotypes. Compare two biological replicates from resistant and susceptible genotypes

ORGANISM(S): Cimex lectularius

SUBMITTER: Asela Wijeratne

PROVIDER: E-GEOD-31823 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

RNA-Seq and molecular docking reveal multi-level pesticide resistance in the bed bug.

Mamidala Praveen P Wijeratne Asela J AJ Wijeratne Saranga S Kornacker Karl K Sudhamalla Babu B Rivera-Vega Loren J LJ Hoelmer Andrew A Meulia Tea T Jones Susan C SC Mittapalli Omprakash O

BMC genomics 20120106

<h4>Background</h4>Bed bugs (Cimex lectularius) are hematophagous nocturnal parasites of humans that have attained high impact status due to their worldwide resurgence. The sudden and rampant resurgence of C. lectularius has been attributed to numerous factors including frequent international travel, narrower pest management practices, and insecticide resistance.<h4>Results</h4>We performed a next-generation RNA sequencing (RNA-Seq) experiment to find differentially expressed genes between pesti ...[more]

PMID: 22226239

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Project description:Infectious Pancreatic Necrosis (IPN) is a highly contagious birnavirus disease of farmed salmonid fish, which often causes high levels of morbidity and mortality. A large genetic component underlying resistance to this disease has been previously described for Atlantic salmon (Salmo salar L.), which mediates high mortality rates in some families and zero mortality in others. A global comparison of the gene expression profiles of resistant and susceptible Atlantic salmon fry following challenge with the IPN virus was undertaken. Full sibling salmon fry from two IPNV-resistant and two IPNV-susceptible families were challenged with the virus and sampled at 1 day, 7 days and 20 days post-challenge. Significant viral titre was observed in both resistant and susceptible fish at all timepoints, although generally at higher levels in susceptible fish. Microarray interrogations were performed using a custom-designed, oligonucleotide microarray platform (Agilent) with 44 K probes per slide (Salar_2; Agilent Design ID:025520). The design is lodged with ArrayExpress ( under accession number A-MEXP-2065. Dual-label hybridisations were undertaken, with each experimental sample (Cy3 labelled) being competitively hybridised against a pooled reference control (Cy5 labelled) comprising equimolar amounts from each experimental RNA sample. The interrogations comprised 144 separate hybridisations; 2 genotypes (susceptible, resistant) Ã 2 families for each genotype Ã 2 challenge states (control, challenged) Ã 3 timepoints (1, 7, 20 dpi) Ã 4 biological replicates for resistant (2 from each of two tanks) and 8 biological replicates for susceptible (4 from each of two tanks). A preliminary analysis suggested evidence for a segregating QTL in both the susceptible families and therefore twice as many offspring were screened. It was later established that the evidence for QTL segregation in one of the families was inconclusive and therefore comparisons were made at the family level only. The analyses took the unbalanced design into account.

Dataset Information

Bedbug RNA-seq

Publications

RNA-Seq and molecular docking reveal multi-level pesticide resistance in the bed bug.

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