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ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing

ABSTRACT: We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein. One replicate is included for each of OrR (WT) or SuUR salivary glands.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Terry Orr-Weaver

PROVIDER: E-GEOD-31899 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Developmental control of gene copy number by repression of replication initiation and fork progression.

Sher Noa N Bell George W GW Li Sharon S Nordman Jared J Eng Thomas T Eaton Matthew L ML Macalpine David M DM Orr-Weaver Terry L TL

Genome research 20111116 1

Precise DNA replication is crucial for genome maintenance, yet this process has been inherently difficult to study on a genome-wide level in untransformed differentiated metazoan cells. To determine how metazoan DNA replication can be repressed, we examined regions selectively under-replicated in Drosophila polytene salivary glands, and found they are transcriptionally silent and enriched for the repressive H3K27me3 mark. In the first genome-wide analysis of binding of the origin recognition com ...[more]

PMID: 22090375

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Developmental Control of Gene Copy Number by Repression of Replication Initiation and Fork Progression

Project description:This SuperSeries is composed of the following subset Series: GSE31895: ChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila GSE31896: RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR GSE31897: ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila GSE31898: CGH to ascertain levels of gDNA in third instar salivary glands of various mutant Drosophila GSE31899: ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing GSE33017: Expression profile of third instar larval salivary gland tissue Refer to individual Series

2011-10-17 | E-GEOD-31900 | biostudies-arrayexpress

SG ORC ChIP-Seq experiment

Project description:modENCODE_submission_3253 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: SuUR - Orr-Weaver(official name : SuUR genotype : SuUR mutant outcross : 3 tags : Transposon tag : GFP description : SuUR mutant as maintained by the Orr-Weaver lab. made_by : Orr-Weaver ); Tissue: salivary gland; Developmental Stage: L3 stage wandering larvae; Genotype: SuUR mutant; EXPERIMENTAL FACTORS: read length (base pair 36) ; tissue: salivary glands; Developmental Stage L3 stage wandering larvae; Antibody (target is dORC2); strain: SuUR

2011-03-23 | E-GEOD-28069 | biostudies-arrayexpress

ChIP-Seq of ORC2 bound to third instar salivary gland DNA in WT and mutant Drosophila, analyzed by Illumina sequencing

Project description:We analyzed ORC2 ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from OrR or SuUR Drosophila. Goals were to ascertain the difference in binding profile between salivary glands expressing and not expressing the Supressor of UnderReplication protein.

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Expression profile of third instar larval salivary gland tissue

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2011-10-18 | E-GEOD-33017 | biostudies-arrayexpress

ChIP-Seq of gamma-H2Av bound to third instar salivary gland DNA in WT and SuUR mutant Drosophila; ChIP-Seq of SUUR and Cdc45 bound to egg chamber DNA from early and late stage egg chambers

Project description:We analyzed gamma-H2Av ChIP-Seq from hand dissected salivary glands of wandering third instar larvae from wild-type (OrR) or suppressor of under-replication (SuUR) mutant Drosophila. Goals were to determine the DNA damage profile relative to underreplicated domains. We analyzed SUUR and Cdc45 ChIP-Seq from hand dissected early (stage 10) and late (stage 12/13) egg chambers from adult Drosophila ovaries. The goals was to determine the localization of SUUR relative to replication forks.

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RNAPolII ChIP to find differences between third instar salivary glands of WT and SuUR

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2011-10-17 | E-GEOD-31896 | biostudies-arrayexpress

ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila

2011-10-18 | GSE31897 | GEO

ChIP with anti-H3K27me3 to compare binding in salivary glands of WT and SuUR Drosophila

Project description:ChIP was performed to identify regions of gDNA bound by H3K27me3 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that H3K27me3 binds differently in under-replicated in SuUR mutant third instar salivary glands. Comparison of H3K27me3 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains

2011-10-17 | E-GEOD-31897 | biostudies-arrayexpress

ChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila

Project description:ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands. Comparison of ORC2 ChIP sample relative to input DNA or control IgG pulldown for two different Drosophila strains

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ChIP with anti-orc2 antibody to identify regions of orc binding in third instar salivary glands of WT and SuUR mutant Drosophila

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