Unknown,Transcriptomics,Genomics,Proteomics

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Expression profile of third instar larval salivary gland tissue

ABSTRACT: We use mRNA-seq to transcriptionally profile larval salivary gland tissue from Drosophila third instar larvae. These data provide insights into tissue physiology and can be used to identify tissue specific transcripts. Salivary glands were dissected from 200 wandering third instar larvae and the associated fat body was removed.Salivary glands were transferred to Graces unsupplemented medium on ice prior to RNA extraction with TRIzol reagent. mRNA-seq samples were prepared from 10 ug of total RNA and subject to Illumina based sequencing.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Terry Orr-Weaver 

PROVIDER: E-GEOD-33017 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Developmental control of gene copy number by repression of replication initiation and fork progression.

Sher Noa N   Bell George W GW   Li Sharon S   Nordman Jared J   Eng Thomas T   Eaton Matthew L ML   Macalpine David M DM   Orr-Weaver Terry L TL  

Genome research 20111116 1

Precise DNA replication is crucial for genome maintenance, yet this process has been inherently difficult to study on a genome-wide level in untransformed differentiated metazoan cells. To determine how metazoan DNA replication can be repressed, we examined regions selectively under-replicated in Drosophila polytene salivary glands, and found they are transcriptionally silent and enriched for the repressive H3K27me3 mark. In the first genome-wide analysis of binding of the origin recognition com  ...[more]

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