Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

The transcriptome of Aedes aegypti females from two strains (Chetumal and Rex-D Puerto Rico) either kept on a sugar diet or blood fed

ABSTRACT: Strain variation in Aedes aegypti transcriptome We report RNA-seq analyses of transcriptional changes across two Ae. aegypti strains

ORGANISM(S): Aedes aegypti

SUBMITTER: William Dunn

PROVIDER: E-GEOD-32074 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Strain Variation in the Transcriptome of the Dengue Fever Vector, Aedes aegypti.

Bonizzoni Mariangela M Dunn W Augustine WA Campbell Corey L CL Olson Ken E KE Marinotti Osvaldo O James Anthony A AA

G3 (Bethesda, Md.) 20120101 1

Studies of transcriptome dynamics provide a basis for understanding functional elements of the genome and the complexity of gene regulation. The dengue vector mosquito, Aedes aegypti, exhibits great adaptability to diverse ecological conditions, is phenotypically polymorphic, and shows variation in vectorial capacity to arboviruses. Previous genome sequencing showed richness in repetitive DNA and transposable elements that can contribute to genome plasticity. Population genetic studies revealed ...[more]

PMID: 22384387

Similar Datasets

The transcriptome of Aedes aegypti (Liverpool strain) females kept on a sugar diet versus those blood-fed

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding. Comparison of the transcriptome of Aedes aegypti females at two physiological conditions and one time point.

2011-03-01 | E-GEOD-24872 | biostudies-arrayexpress

The Adult Aedes aegypti Midgut Peritrophic Matrix Proteome

Project description:This study was undertaken to characterize the proteome of the midgut peritrophic matrix of Aedes aegypti.

2018-04-05 | PXD007627 | Pride

Proteomics Informed by Transcriptomics for Characterising Active Transposable Elements and Genome Annotation in Aedes aegypti

Project description:The incomplete genome annotation of non-model organisms hampers molecular and proteomic studies. Proteomics informed by transcriptomics (PIT) is suited to non-model organisms because peptides are identified using transcriptomic, not genomic, data. Aedes aegypti is the mosquito vector for the (re-)emerging dengue, chikungunya, yellow fever and Zika viruses. An Ae. aegypti genome sequence is available, however experimental evidence for >90% of the Ae. aegypti proteome or the activity of transposable elements (TEs) that constitute 50% of the Ae. aegypti genome is lacking. We used PIT to characterise the proteome of the Aedes aegypti derived cell line Aag2. Hotspots of incomplete genome annotation were identified which are not explained by poor sequence and assembly quality. We developed criteria for the characterisation of proteomically active TEs and demonstrate that protein expression does not correlate with a TE’s genomic abundance. Finally, we identify Phasi Charoen-like virus as an unrecognised contaminant of Aag2 cells. We therefore present the first proteomic characterisation of mobile genetic elements, and provide proof-of-principle that PIT can evaluate a genome’s annotation to guide annotation efforts.

2017-02-15 | PXD003799 | Pride

Dual RNA-seq of parasite and host reveals gene expression dynamics during filarial worm-mosquito interactions

Project description:Parasite biology, by its very nature, cannot be understood without integrating it with that of the host, nor can the host response be adequately explained without considering the activity of the parasite. However, due to experimental limitations, molecular studies of parasite-host systems have been predominantly one-sided investigations focusing on either of the partners. Here we conduct a joint dual RNA-seq time course analysis of filarial parasite and host mosquito to better understand the parasite processes underlying development in, and interaction with, the host tissue from the establishment of infection to the emergence of infective-stage larva. Using the Brugia malayi-Aedes aegypti system, we report the parasite gene transcription dynamics, which exhibit a highly ordered developmental program consisting of a series of cyclical and state-transitioning temporal patterns. And, we contextualize these parasite data in relation to the concurrent dynamics of the host transcriptome. Comparative analyses using uninfected tissues and different host strains reveal the influence of parasite development on the host gene transcription as well as the influence of host environment on the parasite gene transcription. Furthermore, we critically evaluate the life-cycle transcriptome of B. malayi by comparing developmental stages in the mosquito relative to those in the mammalian host, providing insight into gene expression changes underpinning the mosquito-borne parasitic lifestyle of this heteroxenous parasite. Time-course mRNA profiles of filarial parasite Brugia malayi and host mosqutio Aedes aegypti were generated by deep sequencing using Illumina GAIIx.

2014-04-15 | E-GEOD-53664 | biostudies-arrayexpress

Seminal fluid and sperm proteomics of Aedes aegypti

Project description:Sperm samples were extracted from adult A. aegypti male seminal vesicles. Semen samples were extracted from bursa of recently mated bursa of N15 labeled females. The goal was to differentiate between the contribution of seminal fluid proteins and sperm proteins in the semen transfered to the female. Our results yield insights into the molecular function, genome organization, regulation, and evolution of sperm proteins and SFPs in this important disease vector.

2018-12-17 | PXD010293 | Pride

The transcriptome of Aedes aegypti females from two strains (Chetumal and Rex-D Puerto Rico) either kept on a sugar diet or blood fed

Project description:Strain variation in Aedes aegypti transcriptome

2011-11-21 | GSE32074 | GEO

Interactome analysis of Aedes aegypti PIWI proteins

Project description:PIWI-interacting (pi) RNAs are a class of small RNAs that have diverse functions in mosquitoes. In order to uncover novel components involved in biogenesis or function of the piRNA pathway in Aedes aegypti mosquitoes, we performed mass spectrometry analyses on immunoprecipitated PIWI proteins and their interactors.

2021-04-28 | PXD021382 | Pride

Aedes aegypti CLIPB9 activates prophenoloxidase-3 in the presence of CLIPA14 after fungal infection

Project description:Melanization is an integral part of the insect defense system and is often induced by pathogen invasion. Phenoloxidases (POs) are critical enzymes that catalyze melanin formation. PO3 is associated with the antifungal response of the mosquito, Aedes aegypti, but the molecular mechanism of the prophenoloxidase-3 (PPO3) activation is unclear. Here we report that PPO3 cleavage activation is mediated by a clip-domain serine protease, CLIPB9. We purified recombinant CLIPB9 and found that it cleaved PPO3 and increased PO activity in the hemolymph. We then identified CLIPA14 (a serine protease homolog) by co-immunoprecipitation using anti-CLIPB9 antibody. After being cleaved by CLIPB9, Ae. aegypti CLIPA14 acted as a cofactor for PPO3 activation. In addition, dsRNA co-silencing of CLIPB9 and CLIPA14 genes reduced melanization after infection with the entomopathogen, Beauveria bassiana, making the adult mosquitoes more sensitive to fungal infection. These results illustrate the roles of CLIPB9 and CLIPA14 in the PPO activation pathway and revealed the complexity of the upstream serine protease network controlling melanization.

2022-07-21 | PXD033507 | Pride

Mass spectometry Raw data DENV-Aedes aegypti SG experiment

Project description:We studied potential interactions between dengue viral particles and Aedes aegypti mosquito salivary proteins by mass spectometry

2021-05-12 | PXD024959 | Pride

The transcriptome of Aedes aegypti (Liverpool strain) females kept on a sugar diet versus those blood-fed

Project description:We report the RNA-seq based analyses of the transcriptional changes in the Aedes aegypti transcriptome 5 hours after blood feeding.

2011-03-01 | GSE24872 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data