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MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]

ABSTRACT: Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts. MIWI-associated piRNAs from different genotypes were sequenced. Total RNA from purified round spermatids were subjected to Ribozero purification and strand-specific RNAseq lib prepared. Global 5' RACE library was prepare from indicated genotypes.

ORGANISM(S): Mus musculus

SUBMITTER: Ramesh Pillai

PROVIDER: E-GEOD-32184 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Miwi catalysis is required for piRNA amplification-independent LINE1 transposon silencing.

Reuter Michael M Berninger Philipp P Chuma Shinichiro S Shah Hardik H Hosokawa Mihoko M Funaya Charlotta C Antony Claude C Sachidanandam Ravi R Pillai Ramesh S RS

Nature 20111127 7376

Repetitive-element-derived Piwi-interacting RNAs (piRNAs) act together with Piwi proteins Mili (also known as Piwil2) and Miwi2 (also known as Piwil4) in a genome defence mechanism that initiates transposon silencing via DNA methylation in the mouse male embryonic germ line. This silencing depends on the participation of the Piwi proteins in a slicer-dependent piRNA amplification pathway and is essential for male fertility. A third Piwi family member, Miwi (also known as Piwil1), is expressed in ...[more]

PMID: 22121019

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MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing

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2011-11-30 | E-GEOD-32183 | biostudies-arrayexpress

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An Ancient Transcription Factor Initiates the Burst of piRNA Production During Early Meiosis in Mouse Testes (RNA-Seq)

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