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Mili-IP, Miwi-IP, and total RNA from mouse adult testis

ABSTRACT: We deep-sequenced small RNAs after immunoprecipitation of Mili or Miwi, as well as total small RNA from adult mouse testis. The goal of this experiment is to more deeply characterize the piRNA pool from adult mouse testes, using the Illumina platform. Comparison of 2 IP libraries with a non-IP library

ORGANISM(S): Mus musculus

SUBMITTER: Nicolas Robine

PROVIDER: E-GEOD-19172 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A broadly conserved pathway generates 3'UTR-directed primary piRNAs.

Robine Nicolas N Lau Nelson C NC Balla Sudha S Jin Zhigang Z Okamura Katsutomo K Kuramochi-Miyagawa Satomi S Blower Michael D MD Lai Eric C EC

Current biology : CB 20091201 24

<h4>Background</h4>Piwi-interacting RNAs (piRNAs) are approximately 24-30 nucleotide regulatory RNAs that are abundant in animal gonads and early embryos. The best-characterized piRNAs mediate a conserved pathway that restricts transposable elements, and these frequently engage a "ping-pong" amplification loop. Certain stages of mammalian testis also accumulate abundant piRNAs of unknown function, which derive from noncoding RNAs that are depleted in transposable element content and do not engag ...[more]

PMID: 20022248

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