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Genome-wide maps of polyadenylation sites in OPMD-model and control mice

ABSTRACT: We applied deep-sequencing based technique, 3'-Seq, to obtain comprehansive maps of poly-A sites in OPMD-model mice expressing trePABPN1(A17) mutant and control mice Examination of poly-A sites in control and OPMD-model mice (in two ages: 20 weeks and 12 months)

ORGANISM(S): Mus musculus

SUBMITTER: Ran Elkon

PROVIDER: E-GEOD-32302 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The poly(A)-binding protein nuclear 1 suppresses alternative cleavage and polyadenylation sites.

Jenal Mathias M Elkon Ran R Loayza-Puch Fabricio F van Haaften Gijs G Kühn Uwe U Menzies Fiona M FM Oude Vrielink Joachim A F JA Bos Arnold J AJ Drost Jarno J Rooijers Koos K Rubinsztein David C DC Agami Reuven R

Cell 20120412 3

Alternative cleavage and polyadenylation (APA) is emerging as an important layer of gene regulation. Factors controlling APA are largely unknown. We developed a reporter-based RNAi screen for APA and identified PABPN1 as a regulator of this process. Genome-wide analysis of APA in human cells showed that loss of PABPN1 resulted in extensive 3' untranslated region shortening. Messenger RNA transcription, stability analyses, and in vitro cleavage assays indicated enhanced usage of proximal cleavage ...[more]

PMID: 22502866

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