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Unknown,Transcriptomics,Genomics,Proteomics

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Genome-wide maps of polyadenylation sites in control and PABPN1kd cells

ABSTRACT: We applied deep-sequencing based technique, 3'-Seq, to obtain comprehansive maps of poly-A sites in human cells. 3'-Seq was applied to two cell lines (U2OS and RPE-1), in control and PABPN1 knockdown cells Examination of poly-A sites in control and PABPN1kd cells (in two different cell lines)

ORGANISM(S): Homo sapiens

SUBMITTER: Ran Elkon

PROVIDER: E-GEOD-27452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The poly(A)-binding protein nuclear 1 suppresses alternative cleavage and polyadenylation sites.

Jenal Mathias M Elkon Ran R Loayza-Puch Fabricio F van Haaften Gijs G Kühn Uwe U Menzies Fiona M FM Oude Vrielink Joachim A F JA Bos Arnold J AJ Drost Jarno J Rooijers Koos K Rubinsztein David C DC Agami Reuven R

Cell 20120412 3

Alternative cleavage and polyadenylation (APA) is emerging as an important layer of gene regulation. Factors controlling APA are largely unknown. We developed a reporter-based RNAi screen for APA and identified PABPN1 as a regulator of this process. Genome-wide analysis of APA in human cells showed that loss of PABPN1 resulted in extensive 3' untranslated region shortening. Messenger RNA transcription, stability analyses, and in vitro cleavage assays indicated enhanced usage of proximal cleavage ...[more]

PMID: 22502866

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PABPN1 role in regulation of alternative cleavage and polyadenylation

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