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Mouse lung tumor tissue: Dnmt3a KO vs. Dnmt3a WT

ABSTRACT: Transcriptional profiling of mouse lung tumors comparing Dnmt3a KO/K-ras G12D mutant with Dnmt3a WT/K-ras G12D mutant. The goal is to search for the difference of mRNA abundance between Dnmt3a KO and WT tumors. Two-condition experiment, KO vs. WT tissure. Biological replicates: 12.

ORGANISM(S): Mus musculus

SUBMITTER: Qing Gao

PROVIDER: E-GEOD-32415 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deletion of the de novo DNA methyltransferase Dnmt3a promotes lung tumor progression.

Gao Qing Q Steine Eveline J EJ Barrasa M Inmaculada MI Hockemeyer Dirk D Pawlak Mathias M Fu Dongdong D Reddy Seshamma S Bell George W GW Jaenisch Rudolf R

Proceedings of the National Academy of Sciences of the United States of America 20111019 44

Alterations in DNA methylation have been associated with genome-wide hypomethylation and regional de novo methylation in numerous cancers. De novo methylation is mediated by the de novo methyltransferases Dnmt3a and 3b, but only Dnmt3b has been implicated in promoting cancer by silencing of tumor-suppressor genes. In this study, we have analyzed the role of Dnmt3a in lung cancer by using a conditional mouse tumor model. We show that Dnmt3a deficiency significantly promotes tumor growth and progr ...[more]

PMID: 22011581

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