Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse oocyte at primary follicle stage

ABSTRACT: oocytes were collected from ovarys of 6 day old female mice. Targets from four biological replicates of each were generated and the expression profiles were determined using Affymetrix MOE430 A & B. Experiment Overall Design: 4 oocyte at primary follicle stage biological replicates were analyzed

ORGANISM(S): Mus musculus

SUBMITTER: Hua Pan

PROVIDER: E-GEOD-3291 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcript profiling during mouse oocyte development and the effect of gonadotropin priming and development in vitro.

Pan Hua H O'brien Marilyn J MJ Wigglesworth Karen K Eppig John J JJ Schultz Richard M RM

Developmental biology 20051005 2

The molecular basis for acquisition of meiotic and developmental competence, the two main outcomes of oocyte development and essential for producing an egg capable of being fertilized and supporting development to term, is largely unknown. Using microarrays, we characterized global changes in gene expression in oocytes derived from primordial, primary, secondary, small antral, and large antral follicles and used Expression Analysis Systematic Explorer (EASE) to identify biological and molecular ...[more]

PMID: 16168984

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Project description:Dramatic changes in chromatin structure and histone modification occur during oocyte growth, as well as a global cessation of transcription. The role of histone modifications in these processes is poorly understood. We report the effect of conditionally deleting Hdac1 and Hdac2 on oocyte development. Deleting either gene has little or no effect on oocyte development, whereas deleting both genes results in follicle development arrest at the secondary follicle stage. This developmental arrest is accompanied by substantial perturbation of the transcriptome and a global reduction in transcription even though histone acetylation is markedly increased. There is no apparent change in histone repressive marks but there is a pronounced decrease in histone H3K4 methylation, an activating mark. The decrease in H3K4 methylation is likely due to increased expression of Kdm5b because RNAi-mediated targeting of Kdm5b in double mutant oocytes results in an increase in H3K4 methylation. An increase in TRP53 acetylation also occurs in mutant oocytes and may contribute to the observed increased incidence of apoptosis. Taken together, these results suggest seminal roles of acetylation of histone and non-histone proteins in oocyte development. We used microarrays to detail the global programme of gene expression underlying oocyte development and identified distinct classes of regulated genes during this process. Total RNA was extracted from 80 oocytes isolated from mice 12 days-of-age using the PicoPure RNA kit (Arcturus), amplified with the Ovation Pico WTA system (NuGen), and then fragmented and labeled with the FL-OvationTM cDNA Biotin Module V2 (NuGen). Four independent biological replicates were hybridized to Affymetrix GeneChip Mouse 1.1 ST microarrays (http://www.affymetrix.com/). Raw microarray data were analyzed as previously described using MAS5, GeneSpring v7, SAM and EASE software (56). A 1.4-fold cutoff was used for EASE analysis; four biological replicates provide sufficient statistical power and confidence to detect a 1.4-fold change in transcript abundance

Dataset Information

Transcription profiling of mouse oocyte at primary follicle stage

Publications

Transcript profiling during mouse oocyte development and the effect of gonadotropin priming and development in vitro.

Similar Datasets

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