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Effects of the JADE-HBO1 complex on gene expression

ABSTRACT: We find that 499 genes are up-regulated and 457 are down-regulated in response to over-expression of JADE1, while 397 genes are up-regulated and 385 are down-regulated after HBO1 knock-down. For each condition - HBO1 siRNA treatment or JADE1 over-expression - two biological replicates were analyzed in duplicate.

ORGANISM(S): Homo sapiens

SUBMITTER: Eric Paquet

PROVIDER: E-GEOD-33220 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Conserved molecular interactions within the HBO1 acetyltransferase complexes regulate cell proliferation.

Avvakumov Nikita N Lalonde Marie-Eve ME Saksouk Nehmé N Paquet Eric E Glass Karen C KC Landry Anne-Julie AJ Doyon Yannick Y Cayrou Christelle C Robitaille Geneviève A GA Richard Darren E DE Yang Xiang-Jiao XJ Kutateladze Tatiana G TG Côté Jacques J

Molecular and cellular biology 20111205 3

Acetyltransferase complexes of the MYST family with distinct substrate specificities and functions maintain a conserved association with different ING tumor suppressor proteins. ING complexes containing the HBO1 acetylase are a major source of histone H3 and H4 acetylation in vivo and play critical roles in gene regulation and DNA replication. Here, our molecular dissection of HBO1/ING complexes unravels the protein domains required for their assembly and function. Multiple PHD finger domains pr ...[more]

PMID: 22144582

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Effects of the JADE-HBO1 complex on gene expression

Project description:We find that 499 genes are up-regulated and 457 are down-regulated in response to over-expression of JADE1, while 397 genes are up-regulated and 385 are down-regulated after HBO1 knock-down.

2012-03-15 | GSE33220 | GEO

Conserved molecular interactions within MYST-ING acetyltransferase complexes that regulate cell proliferation

Project description:This SuperSeries is composed of the following subset Series: GSE33007: Genome-wide map of HBO1 in cancer derived human cell line GSE33220: Effects of the JADE-HBO1 complex on gene expression Refer to individual Series

2012-03-15 | E-GEOD-33221 | biostudies-arrayexpress

Proteogenomics analysis reveals specific genomic orientations at distal regulatory regions composed by non-canonical histone variants

Project description:Histone variants (H3.1, H3.3, H2A, and macroH2A) were studied about their proteomic and genomic distribution in Hela cells HeLa S3 cells stably expressing either FLAG-tagged H3.1, and H3.3 were grown in suspension in Joklik media containing 10% newborn calf serum (Hyclone), 1% GlutaMAX (Invitrogen), and 1% penicillin-streptomycin, and cells were harvested at log phase. Nuclei were isolated, mononucleosomes were subsequently obtained from these cell lines. Cells were lysed in hypotonic TMSD buffer to isolate nuclei, which were then digested with micrococcal nuclease. The resulting mononucleosomes were immunoprecipitated with anti-FLAG M2-agarose beads (Sigma) and eluted with FLAG peptide (Sigma)

2015-12-22 | E-GEOD-64652 | biostudies-arrayexpress

SILAC experiment quantifying the proteomics of WT and HBO1-KO HeLa

Project description:Lysine lactylation (Kla) links metabolism and gene regulation and plays a key role in multiple biological processes. We report that HBO1 functions as a lysine lactyltransferase to regulate transcription. Quantitative proteomic analysis further revealsed 95 endogenous Kla sites targeted by HBO1, with the majority located on histones.

2024-04-13 | PXD051415 | Pride

Expression data from human subcutaneous adipocytes

Project description:An Affimetrix GeneChip Human Genome U133 Plus 2.0 Array was used to study the effect of oleoylethnolamide on gene expression. The addition of 0.01 mM oleoylethanolamide (OEA) to human subcutaneous adipocytes significantly affected the expression of 1013 genes, with 556 being up-regulated and 457 being down-regulated.

2014-11-04 | GSE55539 | GEO

HDAC3 preferentially binds to RUNX1 in t(8;21) AML cells and collaborates with RUNX1 to repress AML1-ETO-mediated transcriptional activation

Project description:Human histone deacetylase 3 (HDAC3) plays an important role in gene transcription in diseased human cells, such as leukemia. The t(8;21) chromosomal translocation is one of the most commonly observed genetic abnormalities associated with acute myeloid leukemia. This translocation generates the AML1-ETO fusion protein between the wild-type RUNX1 transcription factor and wild-type ETO transcriptional corepressor. To better understand the role of HDAC3 in t(8;21) leukemogenesis, the human HDAC3-containing complexes were isolated from stably-transfected HeLa cells by using anti-FLAG immunoprecipitation. The resulting complexes were resolved in SDS-PAGE. The components of the complexes were identified using LC-MS/MS. We report here that the human RUNX1 transcription is a component of the HDAC3 complexes. We demonstrate that HDAC3 and RUNX1 collaboratively repress AML1-ETO-mediated transcription. These results reveal new insight into how AML1-ETO, RUNX1, and HDAC3 crosstalk to deregulate gene transcription in t(8;21) leukemia cells.

2020-02-17 | PXD017230 | Pride

RIP-seq of polyribosomal SLBP RNP

Project description:UV cross-linking and immunoprecipiatation with high throughput sequencing of cytoplasmic SLBP RNP Polyclonal Î±-SLBP antibody was used to immunoprecipitate SLBP RNP from polyribosomal fractions of HeLa S3 lysates. Mock immunoprecipitation were also performed and serve as a negative control.

2015-12-16 | E-GEOD-62076 | biostudies-arrayexpress

Histone acetylation by HBO1 activates Wnt/β-catenin signaling to promote leukemogenesis in B-cell acute lymphoblastic leukemia

Project description:To investigate the underlying mechanism by which HBO1 promotes B-ALL progression, we established NALM-6 cell lines in which HBO1 has been knocked down by shRNA. We then performed gene expression profiling analysis of NALM-6/shHBO1 cells.

2024-05-31 | GSE233678 | GEO

Proteomic analysis of Tmem232-/- and wild type mice sperm

Project description:The sperm were harvested from caudal epididymides of 8-week-old Tmem232-/- and wild-type adult male mice, and subjected to LC-MS/MS analysis.The total proteins of 5,199 were identified and 4,380 of them were quantified. Results showed that 385 differentially expressed proteins were identified including 88 up-regulated and 255 down-regulated genes (Fold Change≥1.5, P<0.05), and group comparisons were presented in a volcano plot.

2024-11-15 | PXD052758 | Pride

Position-dependent alternative splicing activity revealed by global profiling of alternative splicing events regulated by PTB

Project description:This SuperSeries is composed of the following subset Series: GSE23513: Position-dependent alternative splicing activity revealed by global profiling of alternative splicing events regulated by PTB (HJAY) GSE23514: Position-dependent alternative splicing activity revealed by global profiling of alternative splicing events regulated by PTB (Exon array) Refer to individual Series

2010-09-01 | E-GEOD-23522 | biostudies-arrayexpress

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