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Transcription profiling by array of human benign prostate, primary and metastatic prostate cancer samples to reveal signatures of metastatic progression

ABSTRACT: An integrative analysis of this compendium of proteomic alterations and transcriptomic data was performed revealing only 48-64% concordance between protein and transcript levels. Importantly, differential proteomic alterations between metastatic and clinically localized prostate cancer that mapped concordantly to gene transcripts served as predictors of clinical outcome in prostate cancer as well as other solid tumors. Keywords: prostate cancer progression 13 individual benign prostate, primary and metastatic prostate cancer samples and 6 pooled samples from benign,primary or metastatic prostate cancer tissues.

ORGANISM(S): Homo sapiens

SUBMITTER: Jianjun Yu

PROVIDER: E-GEOD-3325 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrative genomic and proteomic analysis of prostate cancer reveals signatures of metastatic progression.

Varambally Sooryanarayana S Yu Jianjun J Laxman Bharathi B Rhodes Daniel R DR Mehra Rohit R Tomlins Scott A SA Shah Rajal B RB Chandran Uma U Monzon Federico A FA Becich Michael J MJ Wei John T JT Pienta Kenneth J KJ Ghosh Debashis D Rubin Mark A MA Chinnaiyan Arul M AM

Cancer cell 20051101 5

Molecular profiling of cancer at the transcript level has become routine. Large-scale analysis of proteomic alterations during cancer progression has been a more daunting task. Here, we employed high-throughput immunoblotting in order to interrogate tissue extracts derived from prostate cancer. We identified 64 proteins that were altered in prostate cancer relative to benign prostate and 156 additional proteins that were altered in metastatic disease. An integrative analysis of this compendium o ...[more]

PMID: 16286247

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