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Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines

ABSTRACT: We utilized the Barley1 Affymetrix GeneChip for comparative transcript analysis of Betzes barley, Chinese Spring wheat, and Chinese Spring–Betzes ditelosomic chromosome addition lines to physically map barley genes to their respective chromosome arm locations. We mapped barley genes to chromosome arms (1HS, 2HS, 2HL, 3HS, 3HL, 4HS, 4HL, 5HS, 5HL, 7HS, and 7HL) based on their transcript levels in the ditelosomic addition lines. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Hatice Bilgic. The equivalent experiment is BB55 at PLEXdb.]

ORGANISM(S): Triticum aestivum Hordeum vulgare

PROVIDER: GSE33400 | GEO | 2011/11/22

SECONDARY ACCESSION(S): PRJNA149141

REPOSITORIES: GEO

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Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines

Project description:We utilized the Barley1 Affymetrix GeneChip for comparative transcript analysis of Betzes barley, Chinese Spring wheat, and Chinese SpringM-bM-^@M-^SBetzes ditelosomic chromosome addition lines to physically map barley genes to their respective chromosome arm locations. We mapped barley genes to chromosome arms (1HS, 2HS, 2HL, 3HS, 3HL, 4HS, 4HL, 5HS, 5HL, 7HS, and 7HL) based on their transcript levels in the ditelosomic addition lines. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Hatice Bilgic. The equivalent experiment is BB55 at PLEXdb.] genotype: CS (Chinese Spring)(3-replications); genotype: Betzes(3-replications); genotype: 1HS(3-replications); genotype: 2HS(3-replications); genotype: 3HS(3-replications); genotype: 3HL(3-replications); genotype: 4HS(3-replications); genotype: 4HL(3-replications); genotype: 5HS(3-replications); genotype: 5HL(3-replications); genotype: 7HS(3-replications); genotype: 7HL(3-replications)

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Microarray of barley genes in wheat-barley chromosome addition lines

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Project description:Effect of high grain protein locus on barley grain protein accumulation. Gene expression levels were analysed in Karl, a low grain protein variety with its near-isogenic line 10_11(has high grain protein locus, chromosome 6)using Barley1 22k affymetrix chip. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Aravind Jukanti. The equivalent experiment is BB53 at PLEXdb.]

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Project description:The analysis of gene expression during wheat development: Gene expression measurements were carried out on a developmental tissue series for wild-type wheat (cv. Chinese Spring) using the Affymetrix Wheat GeneChip. Thirteen tissues at defined developmental stages were chosen to match the barley (cv. Morex) tissue series of Druka et al. 2006 that used the Affymetrix Barley1 GeneChip. Three replicates of: root tissue at two different developmental stages, leaf, crown, caryopsis, anther, pistil, inflorescence, bracts, mesocotyl, endosperm, embryo and coleoptiles were hybridised. Comparisons between this wheat data and the barley dataset were performed and are available at http://contigcomp.acpfg.com.au [PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Tim Sutton. The equivalent experiment is TA3 at PLEXdb.]

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Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines

Project description:Mapping barley genes to chromosome arms by transcript profiling of wheat-barley ditelosomic chromosome addition lines

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2011-11-22 | GSE33406 | GEO

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