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Microarray analysis of male strobili in fertile and sterile male strobili of Cryptomeria japonica

ABSTRACT: Investigation of comprehensive gene expression level changes in male strobili of sterile mutant, compared to the fertile plant. We designed a new custum array derived from ESTs of Cryptomeria japonica for this study. Male strobili of C. japonica were collected from Toyama 1 half-sib F2 pedigree. Toyama 1 male-sterile mutant was first reported in Saito, M. , H. Taira, and Y. Furuta (Journal of Forest Research 3(3):167-173). Microarray studies using both individual samples and pooled samples were performed. Total RNA was extracted from male strobili at four developmental stages derived from each seven fertile and male-sterile trees as individual samples. Pooled RNA samples were prepared from four to eight different fertile and sterile plants at three developmental stages.

ORGANISM(S): Cryptomeria japonica

SUBMITTER: Norihiro Futamura

PROVIDER: E-GEOD-33499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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