Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Anastasios Mastrokolias
PROVIDER: E-GEOD-33701 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA.
Mastrokolias Anastasios A den Dunnen Johan T JT van Ommen Gertjan B GB 't Hoen Peter A C PA van Roon-Mom Willeke M C WM
BMC genomics 20120118
<h4>Background</h4>Transcriptome analysis is of great interest in clinical research, where significant differences between individuals can be translated into biomarkers of disease. Although next generation sequencing provides robust, comparable and highly informative expression profiling data, with several million of tags per blood sample, reticulocyte globin transcripts can constitute up to 76% of total mRNA compromising the detection of low abundant transcripts. We have removed globin transcri ...[more]
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