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Transcription profiling of diurnal gene expression in Arabidopsis thaliana Col-0 rosette leaves

ABSTRACT: How do the transcript levels of leaf-expressed genes change in a normal day-night cycle? The interest is in genes that are regulated by the circadian clock and the diurnal component (i.e. light, metabolite changes). Plants were grown on soil in a 12/12 h light/dark rythm at 20Â°C day and night. 5 weeks after germination the rosettes of the non-flowering plants were harvested, 15 plants per sample. Plants were harvested at 6 timepoints every 4 hours beginning with the end of the night (still in darkness).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Oliver Blasing

PROVIDER: E-GEOD-3416 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Sugars and circadian regulation make major contributions to the global regulation of diurnal gene expression in Arabidopsis.

Bläsing Oliver E OE Gibon Yves Y Günther Manuela M Höhne Melanie M Morcuende Rosa R Osuna Daniel D Thimm Oliver O Usadel Björn B Scheible Wolf-Rüdiger WR Stitt Mark M

The Plant cell 20051118 12

The diurnal cycle strongly influences many plant metabolic and physiological processes. Arabidopsis thaliana rosettes were harvested six times during 12-h-light/12-h-dark treatments to investigate changes in gene expression using ATH1 arrays. Diagnostic gene sets were identified from published or in-house expression profiles of the response to light, sugar, nitrogen, and water deficit in seedlings and 4 h of darkness or illumination at ambient or compensation point [CO(2)]. Many sugar-responsive ...[more]

PMID: 16299223

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