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Differential RNAs in the sperm cells of asthenozoospermic patients

ABSTRACT: Alterations in the presence of sperm RNAs have been identified using microarrays in teratozoospermic (abnormal morphology) or other types of infertile patients. However, so far no studies had been reported on the sperm RNA content using microarrays in asthenozoospermic patients (low motility). We started the present project to with the goal to characterize the RNA expression in asthenozoospermic infertile patients as compared to normozoospermic fertile controls. We selected four normal fertile donors and four severe asthenozoospermic infertile patients. Equal amounts of RNA were extracted from the sperm samples, subjected to different quality controls and hybridized to the Affymetrix U133 Plus version 2 arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Rafael Oliva

PROVIDER: E-GEOD-34514 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential RNAs in the sperm cells of asthenozoospermic patients.

Jodar Meritxell M Kalko Susana S Castillo Judit J Ballescà Josep Lluís JL Oliva Rafael R

Human reproduction (Oxford, England) 20120221 5

<h4>Background</h4>Alterations in RNAs present in sperm have been identified using microarrays in teratozoospermic patients and in other types of infertile patients. However, so far, there have been no reports on using microarrays to determine the RNA content of sperm from asthenozoospermic patients.<h4>Methods</h4>We started the present project with the goal of characterizing the RNA abundance in the sperm cells of asthenozoospermic patients when compared with controls. To reach this objective, ...[more]

PMID: 22353264

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