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Cigarette smoking alters mRNA expression in human alveolar macrophages

ABSTRACT: Alveolar macrophages from never smokers and active smokers were isolated by bronchoalveolar lavage and gene expression was measured. Chronic cigarette smoke exposure, as occurs in smoker's lungs, leads to significant changes in gene expression. Of note, RNA was isolated immediately following bronchoscopy. Alveolar macrophage levels were >95%. 4 never smokers and 4 active smokers were recruited for the study. After bronchoalveolar lavage and RNA isolation, gene expression was measured using Affymetrix Human Exon 1.0 ST arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Thomas Bair

PROVIDER: E-GEOD-34517 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Cigarette smoking decreases global microRNA expression in human alveolar macrophages.

Graff Joel W JW Powers Linda S LS Dickson Anne M AM Kim Jongkwang J Reisetter Anna C AC Hassan Ihab H IH Kremens Karol K Gross Thomas J TJ Wilson Mary E ME Monick Martha M MM

PloS one 20120829 8

Human alveolar macrophages are critical components of the innate immune system. Cigarette smoking-induced changes in alveolar macrophage gene expression are linked to reduced resistance to pulmonary infections and to the development of emphysema/COPD. We hypothesized that microRNAs (miRNAs) could control, in part, the unique messenger RNA (mRNA) expression profiles found in alveolar macrophages of cigarette smokers. Activation of macrophages with different stimuli in vitro leads to a diverse ran ...[more]

PMID: 22952876

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