Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Cavin1 Deficiency Alters Lung macrophage homeostasis in Mouse

ABSTRACT: Caveolae are cell membrane invaginations that are highly abundant in adipose tissue, endothelial cells and lung and are present at lower levels in other tissues. The formation of caveolae is dependent of the expression of various structural proteins that serve as scaffolding for these membrane invaginations. Cavin1 is a newly identified structural protein whose deficiency leads to absence of caveolae formation and to the development of a lipodystrophic phenotype. In this study we show Cavin1 expression is critical for regulating macrophage number and gene-expression (phenotype) in the lung. We used microarrays to detail the global programme of gene expression in alveolar macrophage in a Cavin1 knock-out mouse and identified distinct classes of dysregulated genes during this process. Bronchoalveolar Lavage performed though a 22-gauge needle tied in place to trachea with PBS containing 0.5 mM ethylenediaminetetraacetic acid (EDTA). Aliquots of 0.5 to 1 ml were instilled into the lungs under direct observation to ensure that all segments of lung were inflated, and aspirated back into the syringe. This was repeated five times per mouse. RNA was extracted from extracted alveolar macrophages and hybridized to Affymetrix microarrays for gene expression analysis.

ORGANISM(S): Mus musculus

SUBMITTER: Adam Gower

PROVIDER: E-GEOD-42169 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cavin1; a regulator of lung function and macrophage phenotype.

Govender Praveen P Romero Freddy F Shah Dilip D Paez Jesus J Ding Shi-Ying SY Liu Libin L Gower Adam A Baez Elizabeth E Aly Sherif Shawky SS Pilch Paul P Summer Ross R

PloS one 20130425 4

Caveolae are cell membrane invaginations that are highly abundant in adipose tissue, endothelial cells and the lung. The formation of caveolae is dependent on the expression of various structural proteins that serve as scaffolding for these membrane invaginations. Cavin1 is a newly identified structural protein whose deficiency in mice leads to loss of caveolae formation and to development of a lipodystrophic phenotype. In this study, we sought to investigate the functional role of Cavin1 in the ...[more]

PMID: 23634221

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Dataset Information

Cavin1 Deficiency Alters Lung macrophage homeostasis in Mouse

Publications

Cavin1; a regulator of lung function and macrophage phenotype.

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