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Effects of water restriction on gene expression in mouse renal medulla

ABSTRACT: In order to identify novel gene targets of vasopressin regulation in the renal medulla, we performed a cDNA microarray study on the inner medullary tissue of mice following a 48 hour water restriction protocol. Three renal medulla of 3 water restricted (WR) mice and three control mice (C) were hybridized against each other such that each WR sample was hybridized against each C sample.

ORGANISM(S): Mus musculus

SUBMITTER: Heddwen Brooks

PROVIDER: E-GEOD-3498 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effects of water restriction on gene expression in mouse renal medulla: identification of 3betaHSD4 as a collecting duct protein.

Cai Qi Q Keck Maggie M McReynolds Matthew R MR Klein Janet D JD Greer Kevin K Sharma Kumar K Hoying James B JB Sands Jeff M JM Brooks Heddwen L HL

American journal of physiology. Renal physiology 20060214 1

To identify novel gene targets of vasopressin regulation in the renal medulla, we performed a cDNA microarray study on the inner medullary tissue of mice following a 48-h water restriction protocol. In this study, 4,625 genes of the possible approximately 12,000 genes on the array were included in the analysis, and of these 157 transcripts were increased and 63 transcripts were decreased by 1.5-fold or more. Quantitative, real-time PCR measurements confirmed the increases seen for 12 selected tr ...[more]

PMID: 16478974

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