Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Human peripheral IL-10-secreting vs non- IL-10-secreting B cells

ABSTRACT: Transcriptional profiling of human peripheral B cell subsets sorted by flow cytometry based on the secretion of IL-10 in response to CpG2006 stimulation. Goal was to identify molecular markers to distinguish IL-10-producing B cells form non-IL-10-producing B cells. Two-condition experiment, non-IL-10-secreting B cells vs. IL-10-secreting B cells. Healthy donors. Biological replicates: 6

ORGANISM(S): Homo sapiens

SUBMITTER: Willem van de Veen

PROVIDER: E-GEOD-35002 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses.

van de Veen Willem W Stanic Barbara B Yaman Görkem G Wawrzyniak Marcin M Söllner Stefan S Akdis Deniz G DG Rückert Beate B Akdis Cezmi A CA Akdis Mübeccel M

The Journal of allergy and clinical immunology 20130226 4

<h4>Background</h4>IL-10-producing regulatory B cells suppress immune responses, and lack of these cells leads to exacerbated symptoms in mouse models of chronic inflammation, transplantation, and chronic infection. IgG4 is a blocking antibody isotype with anti-inflammatory potential that is induced in human high-dose antigen tolerance models.<h4>Objective</h4>We sought to characterize human inducible IL-10-secreting B regulatory 1 (BR1) cells and to investigate their immunoregulatory capacity t ...[more]

PMID: 23453135

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Project description:All established protocols for differentiation of mouse and human pluripotent stem cells into specific neural subpopulations generate a considerable cellular heterogeneity that hampers experimental and clinical progress. In order to obtain a homogenous population of neuronal precursor cells and to streamline the differentiation of embryonic stem cells (ESCs), we assessed PSA-NCAM, a surface glycoprotein that is specifically expressed on immature neurons. We developed an optimized strategy for magnetic isolation of PSA-NCAM positive neuronal precursors from differentiated ESC cultures and characterized their neuronal differentiation potential in vitro. PSA-NCAM enrichment at an early step of neural differentiation increased the number of ES cell derived neurons and reduced cellular diversity. Gene expression analysis revealed that mainly genes involved in neuronal activity were over-represented after purification. The in vivo potential of in vitro derived PSA-NCAM+ enriched precursors was functionally characterized by grafting into the forebrain of adult mice. Analysis for several neuronal and glia markers at 10 or 40 days post graft showed a distinct differentiation pattern. While unsorted control cells gave rise to a mixed population composed of immature precursors, early postmitotic neurons or glial cells, the majority of PSA-NCAM+ enriched cells differentiated into NeuN positive neurons. Furthermore, when in contact with the rostral migratory stream, higher numbers of cells integrated into the stream and migrated towards the olfactory bulb when the PSA-NCAM enriched population was grafted. Thus, enrichment of neuronal precursors based on PSA-NCAM expression represents a general and straightforward approach to narrow cellular heterogeneity during neuronal differentiation of pluripotent cells. Two conditions (step 4, step 5), each represented by three biological replicates of control and enriched cells (Cy5); mESC was used as common reference (Cy3)

Dataset Information

Human peripheral IL-10-secreting vs non- IL-10-secreting B cells

Publications

IgG4 production is confined to human IL-10-producing regulatory B cells that suppress antigen-specific immune responses.

Similar Datasets

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