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Detection of circadian gene expression in human epidermal suction blister samples


ABSTRACT: To detect time of day dependent gene expression in human epidermis suction blister samples from 20 healthy subjects were obtained at three different time points throughout the day. RNA from 20 subjects were used to perform whole genome microarray analysis. Microarrays from 19 subjects showed sufficient quality to perform analysis for differential gene expression. We detected significant differential expression levels for several canonical clock genes such as Per1, Per2, Per3, Bmal1 and Rev-Erb_alpha throughout the day. In total we identified 294 genes that showed significant circadian gene expression including several transcription factors and rate limiting enzymes. To our knowledge this is the first study to investigate genome wide circadian gene expression in human epidermis. Suction blisters of human epidermis were obtained by applying a vacuum for approximately 2.5 h. Suction blisters for each subject (19 subjects in total) were harvested at 9.30 am, 2.30 pm and 7.30 pm.

ORGANISM(S): Homo sapiens

SUBMITTER: Florian Spoerl 

PROVIDER: E-GEOD-35635 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Krüppel-like factor 9 is a circadian transcription factor in human epidermis that controls proliferation of keratinocytes.

Spörl Florian F   Korge Sandra S   Jürchott Karsten K   Wunderskirchner Minetta M   Schellenberg Katja K   Heins Sven S   Specht Aljona A   Stoll Claudia C   Klemz Roman R   Maier Bert B   Wenck Horst H   Schrader Annika A   Kunz Dieter D   Blatt Thomas T   Kramer Achim A  

Proceedings of the National Academy of Sciences of the United States of America 20120618 27


Circadian clocks govern a wide range of cellular and physiological functions in various organisms. Recent evidence suggests distinct functions of local clocks in peripheral mammalian tissues such as immune responses and cell cycle control. However, studying circadian action in peripheral tissues has been limited so far to mouse models, leaving the implication for human systems widely elusive. In particular, circadian rhythms in human skin, which is naturally exposed to strong daytime-dependent c  ...[more]

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