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ChIP-seq study of BEAF binding in four Drosophila spcies

ABSTRACT: Insulators are considered as chromosome organizers. BEAF, one of the insulator proteins, is highly conserved in Drosophila speies but also limited to Drosophila spcies. BEAF associates with TSS of active genes. Comparative study of BEAF binding landscapes in four Drosophila species reveals BEAF association with gene pairs, and the results suggest the role of gain or loss of BEAF binding during the speciation of Drosophila species. DNA sample from ChIP for BEAF and input are collected for each of four Drosophila species

ORGANISM(S): Drosophila pseudoobscura

SUBMITTER: Jingping Yang

PROVIDER: E-GEOD-35648 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The BEAF-32 insulator coordinates genome organization and function during the evolution of Drosophila species.

Yang Jingping J Ramos Edward E Corces Victor G VG

Genome research 20120815 11

Understanding the relationship between genome organization and expression is central to understanding genome function. Closely apposed genes in a head-to-head orientation share the same upstream region and are likely to be coregulated. Here we identify the Drosophila BEAF-32 insulator as a cis regulatory element separating close head-to-head genes with different transcription regulation modes. We then compare the binding landscapes of the BEAF-32 insulator protein in four different Drosophila ge ...[more]

PMID: 22895281

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