Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Whole genome transcription profile showing early changes in gene expression in antigen receptor activated B cells

ABSTRACT: To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 1 and 2 hour. Primary B-lymphocytes were purified from mouse spleens and stimulated with IL-4 (Pepro-Tech) and CD40L (R&D Systems) as indicated at 5 ng/ml and 200 ng/ml, respectively. The BCR of stimulated cells was activated by incubation with goat F(abM-bM-^@M-^Y)2 anti-mouse IgM (Southern Biotechnologies) at 2.5 M-NM-

ORGANISM(S): Mus musculus

SUBMITTER: Thomas GrundstrM-CM-6m

PROVIDER: E-GEOD-35748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Negative feedback regulation of antigen receptors through calmodulin inhibition of E2A.

Verma-Gaur Jiyoti J Hauser Jannek J Grundström Thomas T

Journal of immunology (Baltimore, Md. : 1950) 20120511 12

Signaling from the BCR is used to judge Ag-binding strengths of the Abs of B cells. BCR signaling enables the selection for successive improvements in the Ag affinity over an extremely broad range of affinities during somatic hypermutation. We show that the mouse BCR is subject to general negative feedback regulation of the receptor proteins, as well as many coreceptors and proteins in signal pathways from the receptor. Thus, the BCR can downregulate itself, which can enable sensitive detection ...[more]

PMID: 22581853

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Project description:Meningitis is a life-threatening condition characterized by the inflammation of the leptomeningeal membranes surrounding the brain and spinal cord. The term meningitis is an umbrella term and includes several different etiologies. The majorities of meningitis cases are caused by viruses (viral meningitis; VM) and are often associated with low mortality rates and low risk of developing neurological. In contrast, meningitis caused by some viral infections, such as tick-borne encephalitis (TBE), can be life-threatening when left untreated with increased risk of developing neurological sequelae. Acute bacterial meningitis (ABM), however, is one of the leading causes of death due to infectious diseases worldwide and is associated with rapid disease progression, high mortality rates and increased risk of long-term neurological sequelae in survivors. As meningitis is caused by numerous different pathogens, the host-response is typically highly variable and it is currently unknown if different pathogens can introduce specific proteome changes in the cerebrospinal fluid (CSF). In this study we applied DIA-MS to provide novel insights for in-depth understanding of central nervous system functioning and host response during meningitis in a cohort of patients with differential diagnosis of meningitis, to account for variability contributed by different disease-causing pathogens. The results reveal drastic changes in the CSF proteome during meningitis, where in particular a massive increase of neutrophil derived proteins in the CSF correlated with ABM, suggesting that activated neutrophils play a particular role in ABM. Additionally both ABM and VM result in marked reduction of brain-specific proteins in the CSF, which could be indicative of pathophysiological mechanisms leading to brain damage. Furthermore, generation of lasso regression model enables separation of ABM with high sensitivity and specificity, demonstrating that several proteins are required to confidently discriminate between ABM, VM and BM.

Dataset Information

Whole genome transcription profile showing early changes in gene expression in antigen receptor activated B cells

Publications

Negative feedback regulation of antigen receptors through calmodulin inhibition of E2A.

Similar Datasets

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