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Whole genome transcription profile showing early changes in gene expression in antigen receptor activated B cells

ABSTRACT: To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 1 and 2 hour.

ORGANISM(S): Mus musculus

PROVIDER: GSE35748 | GEO | 2012/07/11

SECONDARY ACCESSION(S): PRJNA152091

REPOSITORIES: GEO

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Whole genome transcription profile showing early changes in gene expression in antigen receptor activated B cells

Project description:To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 1 and 2 hour. Primary B-lymphocytes were purified from mouse spleens and stimulated with IL-4 (Pepro-Tech) and CD40L (R&D Systems) as indicated at 5 ng/ml and 200 ng/ml, respectively. The BCR of stimulated cells was activated by incubation with goat F(abM-bM-^@M-^Y)2 anti-mouse IgM (Southern Biotechnologies) at 2.5 M-NM-<g/ml for 3 hour.

2012-07-10 | E-GEOD-35748 | biostudies-arrayexpress

Whole genome transcription profile of antigen receptor activated B cells

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Whole genome transcription profile of antigen receptor activated B cells

Project description:To search for rapid changes in gene expression following BCR activation, we performed DNA microarray analysis of activated splenic B cells with and without anti-IgM treatment for 3 hour. The expression of a remarkably large set of genes differed significantly. Primary B-lymphocytes were purified from mouse spleens and stimulated with IL-4 (Pepro-Tech) and CD40L (R&D Systems) as indicated at 5 ng/ml and 200 ng/ml, respectively. The BCR of stimulated cells was activated by incubation with goat F(abâ)2 anti-mouse IgM (Southern Biotechnologies) at 2.5 Î¼g/ml for 3 hour.

2010-05-08 | E-GEOD-15606 | biostudies-arrayexpress

Whole genome transcription profile of antigen receptor activated B cells expressing wildtype or Calmodulin (CaM) - resistant E12

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2012-07-10 | E-GEOD-35747 | biostudies-arrayexpress

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NFATc1 controls the survival, function and suppressive capacity of B lymphocytes upon B cell receptor stimulation

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Project description:To simulate transient B cell activation that is the likely initiator of T-dependent responses, we examined the molecular and functional consequences of a single-round of immunoglobulin M (IgM) signaling. This form of activation triggered early cytosolic signaling and transcription factor NF-kB activation indistinguishably from conventional continuous IgM cross-linking, but did not induce G1 progression. However, single-round IgM signaling changed the expression of chemokine and chemokine receptor genes implicated in initiating T-dependent responses, as well as accentuated responsiveness to CD40 signaling. Several features of single-round IgM signaling in vitro were recapitulated in B cells after short-term exposure to antigen in vivo. We propose that transient BCR signals prime B cells to receive T cell help by increasing the probability of B-T encounter and creating a cellular environment that is hyper-responsive to CD40 signaling.

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