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MRNA expression data from AG-haESC, E14 and MEF

ABSTRACT: Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes.Here,we report the derivation of haESCs from androgenetic blastocysts. These cells, which we designated AG-haESCs, express classical ESC markers, are pluripotent, and contribute to various tissues including the germline upon injection into diploid blastocysts. We used microarrays to compare the gene expression levels among androgenetic haploid embryonic stem cell lines(AG-haESC) E14 and male mouse embryonic fibroblasts (MEFs) and identified that most paternally imprinted genes were down-regulated and the maternally imprinted genes were up-regulated. To avoid the influence of diploidized cells on the expression profile, we collected samples from FACS of cells at G1/G0 stage by staining Hochest 33342. We used E14,which was a male embryonic stem cell lines, and MEFs isloated from male individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix GeneChip 430 2.0 array.

ORGANISM(S): Mus musculus

SUBMITTER: Linyu Shi 

PROVIDER: E-GEOD-35785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells.

Yang Hui H   Shi Linyu L   Wang Bang-An BA   Liang Dan D   Zhong Cuiqing C   Liu Wei W   Nie Yongzhan Y   Liu Jie J   Zhao Jing J   Gao Xiang X   Li Dangsheng D   Xu Guo-Liang GL   Li Jinsong J  

Cell 20120401 3

Haploid cells are amenable for genetic analysis. Recent success in the derivation of mouse haploid embryonic stem cells (haESCs) via parthenogenesis has enabled genetic screening in mammalian cells. However, successful generation of live animals from these haESCs, which is needed to extend the genetic analysis to the organism level, has not been achieved. Here, we report the derivation of haESCs from androgenetic blastocysts. These cells, designated as AG-haESCs, partially maintain paternal impr  ...[more]

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