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DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

ABSTRACT: We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites. We used round spermatids as control and analysed the DNA methylation profiles of all the cell lines were by RRBS.

ORGANISM(S): Mus musculus

SUBMITTER: Li Yang

PROVIDER: E-GEOD-60075 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:We generated RRBS data to analyse the DNA methylation profiling among WT-AG-haESCs, DKO-AG-haESCs and round spermatids, we found deletion of H19 and Gtl2 DMRs do not change the methylation patterns in AG-haESCs base on all detected CpG sites.

2015-06-29 | GSE60075 | GEO

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:RNA seq result shows that WT-AG-haESCs and DKO-AG-haESCs samples are clustered together using hierarchical cluster both in the all expression genes and imprinting genes. This suggests that DKO of DMRs of H19 and Gtl2 do not change the overall gene expression patterns in AG-haESCs. We used round spermatids as control. Using RNA-seq, profile of all the expression genes and imprinting genes beteween different samples were analysed.

2015-06-29 | E-GEOD-60072 | biostudies-arrayexpress

Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

2015-06-29 | GSE60072 | GEO

CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells)

Project description:The genomic DNA sample of AG-haESCs were compared to the C57BL/6J male mouse kidney by comparative genomic hybridization. The data confirmed that the haploid cells sustained genome integrity. The analysis was performed on a NimbleGen Mouse CGH 3x720K Whole-Genome Tiling Array to analyse the copy number variations in AG-haESCs, and the genomic DNA of C57BL/6J male mouse kidney was used as control, which had the same background with haploid ESCs.

2012-04-26 | E-GEOD-35786 | biostudies-arrayexpress

Mus musculus

Project description:DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

| PRJNA257422 | ENA

CGH analysis of AG-haESCs (androgenetic haploid embryonic stem cells)

2012-04-27 | GSE35786 | GEO

Mus musculus

Project description:Gene expression analysis in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

| PRJNA257423 | ENA

Gene expression analysis and DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-06-29 | E-GEOD-60076 | biostudies-arrayexpress

Project description:Gene expression analysis and DNA methylation profiling in WT-AG-haESCs, DKO-AG-haESCs and round spermatids

| PRJNA257418 | ENA

mRNA expression data from AG-haESC, E14 and MEF

Project description:Haploid cells are amenable for genetic analysis because they contain only one set of chromosomes.Here,we report the derivation of haESCs from androgenetic blastocysts. These cells, which we designated AG-haESCs, express classical ESC markers, are pluripotent, and contribute to various tissues including the germline upon injection into diploid blastocysts. We used microarrays to compare the gene expression levels among androgenetic haploid embryonic stem cell lines(AG-haESC) E14 and male mouse embryonic fibroblasts (MEFs) and identified that most paternally imprinted genes were down-regulated and the maternally imprinted genes were up-regulated. To avoid the influence of diploidized cells on the expression profile, we collected samples from FACS of cells at G1/G0 stage by staining Hochest 33342. We used E14,which was a male embryonic stem cell lines, and MEFs isloated from male individuals as control. Gene expression profiles of all the cell lines were analysed on an Affymetrix GeneChip 430 2.0 array.

2012-04-26 | E-GEOD-35785 | biostudies-arrayexpress

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