Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human mature endothelial and endothelial cell progenitors treated with Lentiviral gene delivery of vMIP-II as an angiogenesis model

ABSTRACT: We engineered a lentiviral gene vector with vMIP-II, transduced both mature endothelial cells and endothelial cell progenitors, and transplanted these gene-modified cells in Matrigel templates as an in vivo angiogenesis model. The results are the first demonstration in a cell transplantation setting that vMIP-II is proangiogenic in vivo and can deliver this function by vector-mediated gene delivery to both mature endothelial cells and progenitors. Experiment Overall Design: 5 samples in total, hybridised to Affymetrix HG-U133A 2.0 GeneChips, comprising 3 HUVEC control samples and 2 HUVEC experimental samples transduced with vMIP-II.

ORGANISM(S): Homo sapiens

SUBMITTER: Sunil Mathan Kurian

PROVIDER: E-GEOD-3601 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Similar Datasets

Project description:Goal of the experiment : Retroviral-mediated gene transfer of the THAP-zinc finger protein THAP1 inhibits endothelial cell proliferation through coordinated repression of critical cell cycle regulators and pRB-E2F target genes. Experimental design: To gain insight into the effects of THAP1 on endothelial cell growth regulatory pathways, we identified THAP1 target genes in primary human endothelial cells using oligonucleotide-based microarray technology. Human umbilical vein endothelial cells (HUVECs) were transduced with pMLV-MCS or pMLV-THAP1 retroviral expression vectors and, after 48h, cells were harvested for isolation of total RNA and preparation of Cy3- or Cy5-labeled cRNA probes, which were hybridized to DNA microarrays that contained 22 000 unique 60-nt oligonucleotide probes representing > 17 000 human genes. Independent microarray experiments were performed after one (#1xTHAP1) or two (#2xTHAP1) consecutive transductions using independent HUVEC primary cell cultures. Dye swap experiments were performed to eliminate the effect of dye bias, and for each gene probe, the data were subjected to statistical analysis to identify those probes for which a significant difference (p value < 0.01) in mean hybridization intensity was observed between HUVECs transduced with pMLV-MCS or pMLV-THAP1 retroviral expression vectors. Among the gene probes demonstrating a significant difference between the two conditions, we selected those whose expression varied in a similar manner in the two independent microarray experiments. . #2xTHAP1 experiment (Microarrays Code Bars 16011521022012 and 16011521022013): comparison of HUVEC-THAP1 and HUVEC-MCS after two consecutive retroviral transductions of HUVECs with pMLV-THAP1 or pMLV-MCS vectors (higher percentage of genes differentially expressed with p value < 0.01; higher folds) #1xTHAP1 experiment (Microarrays Code Bars 16011521025800 and 16011524025685): comparison of HUVEC-THAP1 and HUVEC-MCS after a single retroviral transduction of HUVECs with pMLV-THAP1 or pMLV-MCS vectors (lower percentage of genes differentially expressed with p value < 0.01; lower folds).

Dataset Information

Transcription profiling of human mature endothelial and endothelial cell progenitors treated with Lentiviral gene delivery of vMIP-II as an angiogenesis model

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets