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Gene array analysis of Human Umbilical Vein Endothelial Cells (HUVEC) transduced with lentivirus containing cDNA of NKX2,5 or GATA4 or GFP.

ABSTRACT: The aim of this study was to induce cardiomyogenic conversion of Human Umbilical Vein Endothelial Cells (HUVEC ) by triggering ectopic expression of the cardiac transcription factors Nkx2.5 and GATA4. Cells were cultured without cell myocardial co-culture system and transduced with lentivirus containing coding sequence of Nkx2.5, GATA4 or GFP as control. Cells were cultured in the EBM-2/ EGM-2 medium, 12 days before microarray analysis. We report in this study that over-expression of NKX2.5 and GATA4 in HUVECs stimulates the expression of some specific genes important in the cardiomyogenic differentiation process, leading to partial switch of these cells from the endothelial to the myocardial course. 12 arrays were analysed. Three competitive hybridizations to the arrays were performed in duplicate using each time, two dye-swap following this experimental design: AWA065 : CY5 (HUVEC+NKX2,5) / CY3 (HUVEC) AWA067 : CY5 (HUVEC) / CY3 (HUVEC+NKX2,5) AWA075 : CY5 (HUVEC+NKX2,5) / CY3 (HUVEC) AWA076 : CY5 (HUVEC) / CY3 (HUVEC+NKX2,5) AWA068 : CY5 ((HUVEC) / CY3 (HUVEC+GATA4) AWA070 : CY5 (HUVEC+GATA4) / CY3 ((HUVEC) AWA073 : CY5 (HUVEC+GATA4) / CY3 ((HUVEC) AWA002 : CY5 ((HUVEC) / CY3 (HUVEC+GATA4) AWA066 : CY5 (HUVEC) / CY3 (HUVEC+NKX2.5+GATA4) AWA069 : CY5 (HUVEC+NKX2.5+GATA4) / CY3 (HUVEC) AWA071 : CY5 (HUVEC+NKX2.5+GATA4) / CY3 (HUVEC) AWA074 ; CY5 (HUVEC) / CY3 (HUVEC+NKX2.5+GATA4)

ORGANISM(S): Homo sapiens

SUBMITTER: isabelle vigon

PROVIDER: E-GEOD-37459 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene array analysis of Human Umbilical Vein Endothelial Cells (HUVEC) transduced with lentivirus containing cDNA of NKX2,5 or GATA4 or GFP.

Project description:The aim of this study was to induce cardiomyogenic conversion of Human Umbilical Vein Endothelial Cells (HUVEC ) by triggering ectopic expression of the cardiac transcription factors Nkx2.5 and GATA4. Cells were cultured without cell myocardial co-culture system and transduced with lentivirus containing coding sequence of Nkx2.5, GATA4 or GFP as control. Cells were cultured in the EBM-2/ EGM-2 medium, 12 days before microarray analysis. We report in this study that over-expression of NKX2.5 and GATA4 in HUVECs stimulates the expression of some specific genes important in the cardiomyogenic differentiation process, leading to partial switch of these cells from the endothelial to the myocardial course.

2012-04-21 | GSE37459 | GEO

Transcription profiling of human HUVEC and OVCA429 cell lines treated with recombinant MAGP2

Project description:Identification of signaling events contributing to the effect of recombinant MAGP2 on HUVECs and OVCA429. We used microarrays to identify the signaling events and up-regulated genes associated with MAGP2. Experiment Overall Design: To explore the consequences of recMAGP2-induced signaling in ovarian cancer, microarrays were completed for a series of recMAGP2-treated (n=3) and untreated (n=3) HUVEC and OVCA429 cells. Experiment Overall Design: The supplementary files 'GSE18373_diff_exp_HUVEC.txt' and 'GSE18373_diff_exp_OVCA429.txt' contain differentially expressed genes for Samples GSM458345-GSM458350 and GSM458351-GSM458356, respectively.

2009-10-27 | E-GEOD-18373 | biostudies-arrayexpress

Transcription profiling of human papillary serous ovarian adenocarcinomas and HUVEC and OVCA429 cells lines treated with recombinant MAGP2 - SuperSeries

Project description:This SuperSeries is composed of the following subset Series:; GSE18373: Expression data of HUVEC and OVCA429 with recombinant MAGP2; GSE18520: Whole-genome oligonucleotide expression analysis of papillary serous ovarian adenocarcinomas Experiment Overall Design: Refer to individual Series

2009-10-23 | E-GEOD-18521 | biostudies-arrayexpress

HUVECs with endogenous or overexpressed GPR4 treated with pH 8.4 (control) or pH 6.4 for 5 h

Project description:Acidic tissue microenvironment is commonly found in a variety of pathophysiological conditions. GPR4 is a proton-sensing G protein-coupled receptor that is fully activated by acidic extracellular pH but has lesser activity at the physiological pH 7.4 and minimal activity at more alkaline pH. To determine the effects of GPR4 activation by acidosis on vascular endothelial cells, we examined the global gene expression of the acidosis response in primary human umbilical vein endothelial cells (HUVEC) with varying level of GPR4. HUVEC with endogenous or overexpressed GPR4 level (designated as HUVEC/Vector & HUVEC/GPR4 cells). Two treatment conditions: pH 6.4 vs. pH 8.4 for 5 h. Biological replicates: 4 HUVEC/Vector replicates (pH6.4 vs pH 8.4), and 4 HUVEC/GPR4 replicates (pH6.4 vs pH 8.4).

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Deubiquitinase USP10 regulates Notch signaling in the endothelium

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2019-04-26 | E-MTAB-7774 | biostudies-arrayexpress

P. aeruginosa PAO wildtype Cy3 PA3898 mutant Cy5

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Transcription profiling of human endothelial gene expression under estradiol treatment

Project description:DNA microarrays were used to investigate global gene expression patterns in cultured human umbilical vein endothelial cells (HUVEC) exposed to 1 nmol/L estradiol for 24 hours, compared to control cells. Experiment Overall Design: HUVEC from 9 separate cultures were exposed to control (0.1% ethanol) and 1nmol/L estradiol treatments for 24 h. Total cellular RNA was extracted. Equal amounts of RNA extracted from 3 control cells or 3 estradiol-treated cells obtained from three different cultures were pooled, achieving three biological replicates of the control and three that were treated with estradiol. Therefore, a total number of 6 microarrays were developed.

2009-07-01 | E-GEOD-16683 | biostudies-arrayexpress

LMWF_EC

Project description:ECFCs and HUVECs were washed and placed overnight in EBM-2 + 2% FCS. The next morning, medium was replaced by EBM-2 + 2% FCS + 10 µg/mL LMWF or by EBM-2 + 2% FCS alone. After 6 hours or 24 hours of treatment, cells were washed with PBS, detached with accutase. After washing and centrifugation steps, the pellet was resuspended in RLT buffer (Qiagen, Venlo, Netherlands) containing beta mercaptoethanol (Sigma, St Louis, MO, USA) and stored at -80°C. Total RNA was isolated by using the RNeasy Mini Kit (Qiagen) as recommended by the manufacturer. Total RNA concentration and integrity was assessed by Nanodrop (Thermo Fisher Scientific, Waltham, USA) and by microchip electrophoresis (Agilent technologies, Santa Clara, CA, USA). Total RNA was amplified using a kit from Ambion (Carlsbad, CA, USA) and applied to Human HT-12 expression beadchips (Illumina, San Diego, CA, USA), a genome wide array with 37 804 probes, according to the manufacturer’s instructions. Data were extracted and normalized using GeneSpring GX (Per Chip: Normalize to 50th percentile; Per Gene: Normalize to median). The working lists were created, filtering probes with detection p-values<0.05 for at least half of the arrays. To control for systematic bias, unsupervised hierarchical clustering was performed, showing that samples are segregated according to the treatment groups. Differentially expressed genes with an expression fold change greater than 1.5 fold were selected using the Student test, with a significance p-value<0.05, including Bonferroni and Benjamini Hochberg false discovery detection. Differential expression of the chosen genes was assessed using supervised hierarchical clustering. The interpretation of the resulting gene lists was performed using the Ingenuity® interface. Heatmaps were generated with R. Each dataset was derived from at least four biologically independent replicate samples.

2016-09-25 | E-MTAB-3925 | biostudies-arrayexpress

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2016-04-01 | E-GEOD-78107 | biostudies-arrayexpress

Identification of common endothelial intronic circular RNAs from different vascular beds

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